Method for detecting total heavy metal content in medicinal materials

A technology of heavy metals and medicinal materials, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of quantitative determination of total heavy metals, etc., and achieve the effect of sensitive response, easy operation, and accurate total heavy metal content

Active Publication Date: 2015-09-23
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Problems solved by technology

In recent years, qualitative or semi-quantitative fluorescence detection methods for specific metal ion...

Method used

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  • Method for detecting total heavy metal content in medicinal materials
  • Method for detecting total heavy metal content in medicinal materials
  • Method for detecting total heavy metal content in medicinal materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the establishment of the mathematical model of total heavy metal content detection in medicinal materials

[0051] 1. Solution preparation

[0052] Preparation of fluorescent probe solution: Accurately weigh 0.380 g of fluorescent probe crystal to dissolve it completely in acetonitrile, place it in a 1L volumetric flask, then add acetonitrile to constant volume to obtain a fluorescent probe solution with a concentration of 1.8 mM, and place it in a UV The fluorescent probe solution exhibits green fluorescence under light. Store in the dark at 4°C for later use.

[0053] Preparation of HEPES buffer solution: Accurately weigh 11.915g of HEPES powder, add 40ml of pure water to dissolve it, adjust the pH value to 7.0, and adjust the volume of pure water to 50ml to obtain a HEPES buffer solution with a concentration of 1.0M. Store at 4°C for later use .

[0054]Preparation of lead nitrate solution: Weigh different lead nitrate standard products and dissolve ...

Embodiment 2

[0064] Embodiment 2, the detection of total heavy metal content in Akebia

[0065] 1. Solution preparation

[0066] Probe solution preparation: Precisely weigh 0.380 g of the probe crystal to be completely dissolved in acetonitrile, place it in a 1L volumetric flask, and then add acetonitrile to constant volume to obtain a fluorescent probe solution with a concentration of 1.8 mM. The fluorescent probe solution showed green fluorescence. Store in the dark at 4°C for later use.

[0067] Preparation of HEPES buffer solution: Accurately weigh 11.915g of HEPES powder, add 40ml of pure water to dissolve it, adjust the pH to 7.0, and adjust the volume of pure water to 50ml to obtain a HEPES buffer solution with a concentration of 1.0M. Store at 4°C for later use.

[0068] Preparation of Akebia test solution: Weigh 0.5g of Akebia powder sample, add 50ml of acetonitrile solution to dissolve it, sonicate for 1min and centrifuge to take the supernatant for later use.

[0069] 2. Dete...

Embodiment 3

[0075] Embodiment 3, the detection of total heavy metal content in Magnolia officinalis

[0076] 1. Solution preparation

[0077] Probe solution preparation: Precisely weigh 0.380 g of the probe crystal to be completely dissolved in acetonitrile, place it in a 1L volumetric flask, and then add acetonitrile to constant volume to obtain a fluorescent probe solution with a concentration of 1.8 mM. The fluorescent probe solution showed green fluorescence. Store in the dark at 4°C for later use.

[0078] Preparation of HEPES buffer solution: Accurately weigh 11.915g of HEPES powder, add 40ml of pure water to dissolve it, adjust the pH to 7.0, and adjust the volume of pure water to 50ml to obtain a HEPES buffer solution with a concentration of 1.0M. Store at 4°C for later use.

[0079] Preparation of magnolia bark test solution: Weigh 0.5g magnolia bark powder sample, add 50ml acetonitrile solution to dissolve it, ultrasonicate for 1min, and centrifuge to take the supernatant for ...

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Abstract

The invention discloses a method for detecting the total heavy metal content in medicinal materials. The method comprises the steps of firstly, building a mold for detecting the total heavy metal content in medicinal materials, and obtaining an equation of a linear standard curve I and an equation of a linear standard curve II; then, determining a fluorescence intensity value of a reaction system after medicinal materials to be detected are mixed with an HEPES buffer solution and a fluorescence probe solution to have a reaction; substituting the fluorescence intensity ratio I450/I380 into the equation of the linear standard curve to obtain a metal ion concentration C value in the medicinal material to be detected; substituting the metal ion concentration C value in the medicinal material to be detected into the equation of the linear standard curve II, and obtaining the total metal content in the medicinal material. According to the method, the heavy metal ions in the medicinal material are combined with a fluorescence probe, and the medicinal materials are calibrated through fluorescence; by means of the advantage that the fluorescence probe is flexible and rapid in reaction, the total heavy metal content in the medicinal materials to is accurately detected, operation is easy and convenient and consumed time is short.

Description

technical field [0001] The invention relates to a method for detecting the content of total heavy metals in medicinal materials, in particular to a method for establishing a mathematical model for detecting the content of total heavy metals in medicinal materials by fluorescent probe labeling and a method for detecting the content of total heavy metals in medicinal materials by using the model, belonging to traditional Chinese medicine Material quality inspection field. Background technique [0002] Heavy metals usually refer to specific gravity greater than 5g cm -3 A class of metal elements such as copper, cadmium, gold, silver, lead, zinc, nickel, cobalt, chromium, and mercury. At present, with the acceleration of urbanization and industrialization, environmental pollution is increasing day by day, which increases the content of heavy metals in Chinese medicinal materials, reduces the quality of Chinese medicinal materials, and seriously endangers human health. Heavy me...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 黄璐琦袁媛南铁贵詹志来郑琪
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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