A device for combined use of hollow fiber membrane liquid phase microextraction and liquid chromatography and a method for on-line quantitative analysis of polysaccharide components
A technology of quantitative analysis and extraction device, which is applied to the device combined with hollow fiber membrane liquid phase micro-extraction and liquid chromatography, and the field of on-line quantitative analysis of polysaccharide components, which can solve unseen problems and achieve simple structure, simple and convenient extraction process. control, good application prospects
Active Publication Date: 2017-03-01
丽水智客汇信息服务有限公司
View PDF6 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
After searching, there are no patent disclosures similar to the present invention, and bibliographical reports on quantitative analysis of biological samples carried out with similar experimental devices of the present invention
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0036] A detailed description will be given below of specific embodiments of the present invention.
[0037] Such as figure 1As shown, a hollow fiber membrane liquid-phase micro-extraction liquid chromatography device, the device includes a sampling part 2, a liquid chromatography pump 4, a six-way valve 5, a chromatographic column 6, a detector 7, a sample loop 9 , a waste liquid bottle 10 and an ultrasonic cleaning machine 11, a hollow fiber membrane liquid-phase microextraction device 1 is arranged in the described ultrasonic cleaning machine 11, and a liquid chromatography universal Pipeline 8, one end liquid chromatography common pipeline 8 is connected with described six-way valve 5 and sampling part 2, and described six-way valve 5 is connected with described liquid chromatography pump 4, chromatographic column 6 respectively , the sample loop 9 is connected to the waste liquid bottle 10, the chromatographic column 6 is connected to the detector 7, and the detector 7 i...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| length | aaaaa | aaaaa |
| length | aaaaa | aaaaa |
| length | aaaaa | aaaaa |
Login to View More
Abstract
The invention relates to a hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and a polysaccharide component on-line quantitative analysis method thereof. The device comprises a sample feeding part, a liquid chromatography pump, a six-way valve, a chromatographic column, a detector, a sample ring, a waste liquid bottle and an ultrasonic washer, wherein a hollow-fiber membrane liquid-phase micro-extraction device is arranged inside the ultrasonic washer; general liquid chromatography pipelines are arranged at two ends of the hollow-fiber membrane liquid-phase micro-extraction device; the general liquid chromatography pipeline at one end is connected to the six-way valve and the sample feeding; the six-way valve is respectively connected to the liquid chromatography pump, the chromatographic column, the sample ring and the waste liquid bottle; the chromatographic column is connected to the detector; the detector is connected to the waste liquid bottle. The hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device has the characteristics as follows: samples are efficiently and quickly purified; the method is scientific and reasonable; the extraction conditions are adjustable; the application prospect is good.
Description
technical field [0001] The invention relates to an experimental device for sample pretreatment, in particular to a hollow fiber membrane liquid-phase micro-extraction liquid-phase chromatography device and an online quantitative analysis method for polysaccharide components. Background technique [0002] Polysaccharides are natural polymer compounds that widely exist in nature and are composed of monosaccharides linked by glycosidic bonds. The determination of monosaccharide composition of polysaccharides is the most important link to control the quality standards of polysaccharides and provide basic information of polysaccharides. Monosaccharide composition is a basic and important content of sugar analysis. Since most sugars do not have optical properties, when the sugars are separated and determined by chromatography or electrophoresis, ultraviolet or fluorescent reagents are often used to derivatize the sugars to improve their optical activity and detection sensitivity....
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 王娜妮寿旦费莹
Owner 丽水智客汇信息服务有限公司