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Method for eliciting an immune response to an immunogen

An immune response and immunogen technology, applied in the field of eliciting immune responses to immunogens, can solve problems such as expensive, unrecognized target proteins, and lengthy

Active Publication Date: 2015-09-23
AGENCY FOR SCI TECH & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, there is a high risk that antibodies generated from the isolated peptide may not recognize the target protein in vivo after all
[0007] While transfected cells and lipid-based systems have been used to present membrane protein antigens to increase the chances of isolating antibodies that may be effective in vivo, these systems are often unstable, lengthy and expensive

Method used

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  • Method for eliciting an immune response to an immunogen
  • Method for eliciting an immune response to an immunogen
  • Method for eliciting an immune response to an immunogen

Examples

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Embodiment 1

[0058] A method for eliciting an immune response to α-hemolysin in mice using α-hemolysin presenting polymersomes is now described in the following paragraphs.

[0059] Materials & Methods

[0060] Poly(butadiene-b-ethylene oxide) (PBd 21 -PEO 14 ) BD21 amphiphilic block copolymer was purchased from Polymer Source (Polymer Source, Canada). α-hemolysin was from Staphylococcus aureus, 3-(N-morpholino)propanesulfonic acid (MOPS), tris(hydroxymethyl)aminomethane hydrochloride (Tris), magnesium chloride and sodium chloride were purchased from Sigma Aldrich Corporation (Singapore). Tetrahydrofuran (THF) was purchased from Tedia Corporation (Ohio, USA).

[0061] Preparation of polymersomes

[0062] Polymersomes were prepared by a membrane rehydration method. BD21 polymer was dissolved in THF and dried as a thin film on the wall of a conical bottom glass tube under nitrogen flow. The polymer film was further dried under vacuum. Subsequently, ultrapure water was added to th...

Embodiment 2

[0077] A method for eliciting an immune response in mice using hemagglutinin-presenting polymersomes is now described in the following paragraphs.

[0078] Preparation of hemagglutinin polymersomes

[0079] Polymersomes were prepared by a thin film rehydration method. BD21 polymer was dissolved in chloroform and dried into thin films. Subsequently, ultrapure water was added to rehydrate the membrane and allow the spontaneous formation of polymersomes. The resulting vesicle dispersions were then extruded with 0.45 μm and 0.22 μm membranes and dialyzed against ultrapure water to remove any residual solvent.

[0080] Recombinant hemagglutinin (HA) protein (influenza A virus H3N2 Wisconsin 67 / 05 , MyBioSource) was supplied as a sterile-filtered solution in 10 mM sodium phosphate, pH 7.4, 150 mM NaCl and 0.005% Tween-20.

[0081]HA polymersomes were formed by adding an aliquot of the HA solution to the polymersome dispersion. The mixture is then incubated to allow recombina...

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Abstract

The invention relates to methods for eliciting an immune response to an immunogen, and in particular, to such methods using polymersomes as carriers for the immunogen.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority from Singapore Patent Application No. 201208483-6 filed on 19 November 2012, the contents of which are hereby incorporated by reference in their entirety for all purposes. technical field [0003] The present invention relates to methods for eliciting an immune response to an immunogen, in particular, to such methods using polymersomes as carriers for the immunogen. Background technique [0004] Although immunization is a well-established process, there are differences in the level of response elicited between different immunogens or antigens (used interchangeably herein). Interestingly, membrane proteins form a class of antigens that generate low response levels, which in turn means that large amounts of membrane proteins are required in order to generate or induce an immune response to the required level. Membrane proteins are notoriously difficult to synthesize and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/10
CPCA61K39/12C12N2760/16134A61K2039/6093A61K39/385A61K9/1273A61K2039/55566A61K9/0019A61K47/34A61P37/04
Inventor 马达范·纳拉尼法比安·德卡约傅智康苏杏芳
Owner AGENCY FOR SCI TECH & RES
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