Herpes simplex keratitis vaccine and preparation method thereof

Abstract

The invention discloses a herpes simplex keratitis vaccine and a preparation method thereof, wherein the herpes simplex keratitis vaccine comprises plasmid pRSC-gD.gC-IL-21, is packed by adopting nano chitosan, the gC and gD respectively are one of main envelope glycoproteins of herpes simplex virus I, adopt extracellular region genomes, and IL-21 is immunologic adjuvant. The method for preparing the herpes simplex keratitis vaccine comprises 1 HSV-1gD, gC genes are acquired; 2 pRSC-gC.gD-IL-21 plasmids are built; and 3 chitosan / plasmid deoxyribonucleic acid (DNA) nanovaccines are prepared. The herpes simplex keratitis vaccine can safely and effectively resist infection of herpes simplex virus-1 (HSV-1), obviously restrains HSV to happen and develop, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a herpes simplex virus keratitis vaccine and a preparation method thereof. Background technique [0002] Herpes simplex keratitis (HSK) is a serious worldwide blinding eye disease, mainly caused by herpes simplex virus type I (HSV-1) infection. . The pathogenesis of HSK is mainly divided into primary infection, latent and recurrent infection: the primary infection is that HSV-1 directly infects corneal epithelial cells causing punctate or dendritic keratitis; after primary infection, HSV-1 virus can travel along the nerve axis The process flows backward to the local trigeminal ganglion (TG), forming a latent state. It has been reported that the virus can reach the TG within 24 hours after infection; when the body's immunity is low, the latently infected HSV-1 can be reactivated, causing recurrent infections, mostly manifested as stromal keratitis (SK), Its condition is more serious th...

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Problems solved by technology

The above treatments can alleviate the symptoms of HSK and control the recurrence of HSV-1 infection to a certain extent, but they cannot avoid the onset of HSK, let alone effectively eliminate the virus in the incubation period, and cannot achieve a radical cure.

During the medication period, the virus is easy to fall off and can still cause transmission, and the long-term use of the drug is a great burden on the patient both economically and psychologically; on the other hand, due to the long-term use of antiviral drugs, the drug-resistant strains of the virus Recurring, the curative effect will be affected, and there will be a certain degree of side effects

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