Herpes simplex keratitis vaccine and preparation method thereof

A technology of viral keratitis and herpes simplex virus, which is applied in the field of herpes simplex virus keratitis vaccine and its preparation, can solve the problems of inability to achieve a cure, avoid the onset of HSK, and affect the curative effect, and achieve the effect of great social benefits

Active Publication Date: 2015-09-30
SOUTHEAST UNIV
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  • Abstract
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Problems solved by technology

The above treatments can alleviate the symptoms of HSK and control the recurrence of HSV-1 infection to a certain extent, but they cannot avoid the onset of HSK, let alone effectively eliminate the virus in the incubation period, and cannot achieve a radical cure.
During the medication period, the virus is easy to fall off and can st

Method used

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  • Herpes simplex keratitis vaccine and preparation method thereof
  • Herpes simplex keratitis vaccine and preparation method thereof

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Embodiment Construction

[0050] The present invention will be further described below in combination with specific embodiments.

[0051] The herpes simplex virus keratitis vaccine provided by the present invention is composed of plasmid pRSC-gD gC-IL-21, which is encapsulated by nano-chitosan; wherein, gC and gD are the main envelope glycoproteins of herpes simplex virus type I respectively One of them adopts the genome of the extracellular region, and IL-21 is an immune adjuvant.

[0052] Among them, the concentration of plasmid pRSC-gD·gC-IL-21 was 5 μg / μl.

[0053] The above-mentioned herpes simplex virus keratitis vaccine can be prepared according to the following method:

[0054] Step 1: Acquisition of HSV-1gD; gC gene

[0055] 1.1. Cell and virus culture:

[0056] 1.1.1 Preparation of RPMI 1640 medium;

[0057] 1.1.2 Cell culture: the green monkey kidney passage (Vero) cells were resuscitated and passaged;

[0058] 1.1.3 The cultivation of virus: inoculate HSV-1 virus suspension in Vero cel...

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Abstract

The invention discloses a herpes simplex keratitis vaccine and a preparation method thereof, wherein the herpes simplex keratitis vaccine comprises plasmid pRSC-gD.gC-IL-21, is packed by adopting nano chitosan, the gC and gD respectively are one of main envelope glycoproteins of herpes simplex virus I, adopt extracellular region genomes, and IL-21 is immunologic adjuvant. The method for preparing the herpes simplex keratitis vaccine comprises 1 HSV-1gD, gC genes are acquired; 2 pRSC-gC.gD-IL-21 plasmids are built; and 3 chitosan/plasmid deoxyribonucleic acid (DNA) nanovaccines are prepared. The herpes simplex keratitis vaccine can safely and effectively resist infection of herpes simplex virus-1 (HSV-1), obviously restrains HSV to happen and develop, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a herpes simplex virus keratitis vaccine and a preparation method thereof. Background technique [0002] Herpes simplex keratitis (HSK) is a serious worldwide blinding eye disease, mainly caused by herpes simplex virus type I (HSV-1) infection. . The pathogenesis of HSK is mainly divided into primary infection, latent and recurrent infection: the primary infection is that HSV-1 directly infects corneal epithelial cells causing punctate or dendritic keratitis; after primary infection, HSV-1 virus can travel along the nerve axis The process flows backward to the local trigeminal ganglion (TG), forming a latent state. It has been reported that the virus can reach the TG within 24 hours after infection; when the body's immunity is low, the latently infected HSV-1 can be reactivated, causing recurrent infections, mostly manifested as stromal keratitis (SK), Its condition is more serious th...

Claims

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Application Information

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IPC IPC(8): A61K39/245A61P31/22A61P27/02C12N15/85C12N15/66
Inventor 胡凯
Owner SOUTHEAST UNIV
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