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A recombinant virus vaccine strain expressing African swine fever virus p72 protein

An African swine fever virus, recombinant virus technology, applied in the direction of antiviral agents, virus antigen components, viruses/phages, etc., can solve the problems of strong virus virulence, unfavorable eradication, death, etc., and achieve the effect of effective protection

Active Publication Date: 2018-05-25
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There have been many reports on the research of African swine fever vaccine abroad, but traditional inactivated vaccines cannot produce effective immune protection against African swine fever virus infection
In 1960, during the outbreak of African swine fever in Portugal, a weakened strain was used for immunization prevention, and some immunized pigs became ill or even died. In addition, a large number of infected pigs appeared due to immunization, which was very unfavorable for the later eradication of the disease , and there is a risk of virus virulence becoming stronger after inoculation with attenuated African swine fever vaccine that has been weakened in passage

Method used

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  • A recombinant virus vaccine strain expressing African swine fever virus p72 protein
  • A recombinant virus vaccine strain expressing African swine fever virus p72 protein
  • A recombinant virus vaccine strain expressing African swine fever virus p72 protein

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Embodiment Construction

[0026] Further illustrate the present invention below in conjunction with embodiment, content wherein should not be interpreted as limiting the scope of this invention. The reagents described in the examples are all commercial reagents unless otherwise specified.

[0027] 1 Materials and methods

[0028] 1.1 Materials

[0029]1.1.1 Strains and plasmids: Newcastle disease virus MG7 (its F protein cleavage site is replaced by Lasota F protein cleavage site, artificially weakened strain)

[0030] In the present invention:

[0031] MG7 is the Newcastle disease virus of gene type VII isolated and identified by the inventor's laboratory. Through reverse genetics operation, the F protein cleavage site of G7 is replaced with the cleavage site of Lasota strain F protein, thereby mutating and weakening the virus. The strain is named MG7, which is preserved by our laboratory, and its nucleotide sequence is SEQ ID No.5. In addition, the specific preparation method of this strain has b...

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Abstract

The invention provides a preparation method of a recombinant Newcastle disease virus vaccine strain expressing African swine fever virus p72 protein and the recombinant Newcastle disease virus vaccine strain. The method of the present invention includes: constructing a recombinant transcription plasmid in which the p72 gene of African swine fever virus (ASFV) is inserted, constructing a transcription auxiliary plasmid system, and co-transfecting the above transcription plasmid and the transcription auxiliary plasmid into the attenuated Newcastle disease strain to be able to replicate The host cell BHK‑21 was rescued to obtain the recombinant virus strain. The vaccine strain expressing the African swine fever virus p72 protein prepared by the invention has important reserve and strategic significance in the prevention and control of the animal disease, and can be used in the treatment and prevention of the African swine fever virus.

Description

technical field [0001] The invention provides a preparation method of a recombinant Newcastle disease virus vaccine strain expressing African swine fever virus p72 protein and the recombinant Newcastle disease virus vaccine strain. Background technique [0002] African swine fever (ASF) is an acute, febrile, highly contagious disease caused by the African swine fever virus (ASFV), the only member of the African swine fever virus family [1]. Domestic pigs and European wild boars are generally susceptible, showing complex clinical symptoms, causing congestion, hemorrhage and dysfunction of the digestive and respiratory systems, and is a highly contagious disease in domestic pigs [2-4]. African wild boars usually show subclinical and no clinical symptoms of infection, and are the storage hosts of the disease; members of the genus Ornithodoros erraticus (Ornithodoros erraticus, Ornithodoros moubata) in soft ticks can serve as biological agents of the disease and storage hosts of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/45C12N15/34C12N7/01A61K39/12A61P31/20C12R1/93
Inventor 杨吉飞朱启运陈新新刘志杰关贵全罗建勋殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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