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A high-yielding mutant of Saccharopolyspora chinensis butenyl spinosyn

A technology of polyspora butene and polyspora polyspora, applied in fungi, microorganism-based methods, microorganisms, etc., can solve problems such as unknown sequences and improvement of difficult strains

Active Publication Date: 2018-02-13
湖南鸿健生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The complete genome sequence of Saccharopolyspora mentabilis is unknown and has a strictly restricted modification system, making it difficult to improve strains through genetic recombination

Method used

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  • A high-yielding mutant of Saccharopolyspora chinensis butenyl spinosyn
  • A high-yielding mutant of Saccharopolyspora chinensis butenyl spinosyn
  • A high-yielding mutant of Saccharopolyspora chinensis butenyl spinosyn

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Embodiment 1

[0031] The high-yielding mutant strain of Saccharopolyspora serovars butenyl spinosyn of the present embodiment, S13, Saccharopolyspora pogona S13, the strain was preserved in the China Center for Type Culture Collection (CCTCC for short, address: Wuhan University, Wuhan, China) on May 26, 2015, and the strain preservation number is CCTCC NO: M 2015324.

[0032] (1) Specific medium formula and culture conditions

[0033] (1) Saccharopolyspora pogona Seed activation uses SP-2 medium: glucose 9 g / L, tryptone soybean broth 30 g / L, yeast extract 3 g / L, magnesium sulfate 2 g / L; inoculate monoclonal or 1wt% strain preservation solution Add it to a 150 mL shake flask filled with 20 mL SP-2, and incubate at 30°C and 160 r / min for 48 h;

[0034] (2) The transfer and screening of resistant mutant strains used tryptone soy broth medium (TSB): tryptone soy broth 30 g / L;

[0035] (3) S. pogona Spores were collected using MS medium: mannitol 20 g / L, bean cake powder 20 g / L.

[0036]...

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Abstract

A mutant strain of Saccharopolyspora pogona with high production of butenyl spinosad, Saccharopolyspora pogona S13, was preserved in China Center for Type Culture Collection on May 26, 2015. The deposit number is CCTCC NO: M 2015324. The synthetic ability of butenyl spinosyn in the mutant strain is 0.79 times higher than that of the original strain. The results of mass spectrometry showed that the synthesis of new components of butenyl spinosyn was activated in the mutant strain, and a butenyl spinosyn component, Spinosyn α1, was added in the metabolites of the mutant strain than in the original strain.

Description

technical field [0001] The present invention relates to a high-yielding butenyl pleocidin strain of Saccharopolyspora palpitaciens. Ribosome engineering technology is used to screen the resistance of the original strain with high-concentration streptomycin, and a high-yielding butenyl pleocidin strain is obtained. Streptomycin spontaneous resistance mutant strain S13, and the butenyl spinosyn was detected and identified by ultra-high performance liquid chromatography and mass spectrometry. Background technique [0002] Spinosad and its derivatives, as efficient, environmentally friendly and safe antibiotic biopesticides, have become a hot spot in the field of biopesticide research in recent years. butenyl spinosyn S. pogona A class of macrolide compounds similar in structure to spinosads are produced, which have good insecticidal and acaricidal activities. So far, a total of 31 species of spinosyns have been detected, and the spinosyns have a wider insecticidal spectrum th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P19/62C12R1/645
CPCC12P19/62C12N1/205C12R2001/01
Inventor 夏立秋罗林根杨燕丁学知
Owner 湖南鸿健生物科技有限公司
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