Method for improving regeneration efficiency of wild tallow excellent strain stem

A technology of regeneration efficiency and stem segment, which is applied in the field of plant biology, can solve the problems of unfit for the preservation and reproduction of excellent mother plants of Chinese tallow tree, low reproduction coefficient, and poor repeatability, and achieve the goal of cultivating varieties, high reproduction coefficient and fast regeneration speed Effect

Active Publication Date: 2015-10-21
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, researches on the tissue culture of Chinese tallow tree mainly focus on using the endosperm, leaves, hypocotyls or roots of seedlings as explants to induce buds or callus, or using young Chinese tallow tree embryos as explants to pass through the body. The cell embryogenesis pathway induces the regeneration of Chinese tallow tree plants, however, due to the highly heterozygous genetic background characteristics of Chinese tallow tree, the above-mentioned techniques are not suitable for the preservation and p

Method used

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Effect test

Embodiment 1

[0034] A method for improving the regeneration efficiency of stem segments of Chinese tallow tree excellent strains in the field is characterized in that it comprises the following steps:

[0035] Step 1, take the year-old stem section of the excellent strain of Sapium pachyrhiza in the field as an explant, and the stem section is a non-lignified or semi-lignified stem section, and after preliminary disinfection, carry out surface disinfection in an aseptic operating table; The initial disinfection is to remove the leaves of the stems and put them in 1000ml beakers, rinse them under running water for 10 minutes, add 2ml of washing liquid to rinse them, and shake them constantly. Surface disinfection means that after the initial disinfection of the stem segment is wiped with 75% alcohol, it is cut into small pieces with a length of 4 cm; then soaked in 75% alcohol for 15 seconds, rinsed with sterile water 3 times; then soaked in 75% alcohol 15s, rinsed with sterile water 3 time...

Embodiment 2

[0043] A method for improving the regeneration efficiency of stem segments of Chinese tallow tree excellent strains in the field is characterized in that it comprises the following steps:

[0044] Step 1, take the year-old stem section of the excellent strain of Sapium pachyrhiza in the field as an explant, and the stem section is a non-lignified or semi-lignified stem section, and after preliminary disinfection, carry out surface disinfection in an aseptic operating table; Preliminary disinfection is to remove the leaves of the stems and put them in 1000ml beakers, rinse them under running water for 15 minutes, add 4ml washing liquid to rinse them, and shake them constantly. Surface disinfection means that after the initial disinfection of the stem segment is wiped with 75% alcohol, it is cut into small pieces with a length of 6 cm; then soaked in 75% alcohol for 25 seconds, rinsed with sterile water for 5 times; then soaked in 75% alcohol 25s, rinse with sterile water 5 time...

Embodiment 3

[0052] A method for improving the regeneration efficiency of stem segments of Chinese tallow tree excellent strains in the field is characterized in that it comprises the following steps:

[0053] Step 1, take the year-old stem section of the excellent strain of Sapium pachyrhiza in the field as an explant, and the stem section is a non-lignified or semi-lignified stem section, and after preliminary disinfection, carry out surface disinfection in an aseptic operating table; Preliminary disinfection is to remove the leaves of the stems and put them in 1000ml beakers, rinse them under running water for 12 minutes, add 3ml washing liquid to rinse them, and shake them constantly. Surface disinfection refers to the initial disinfection of the stem segment after being wiped with 75% alcohol, and then cut into small sections with a length of 5 cm; then soaked in 75% alcohol for 20 seconds, rinsed with sterile water 4 times; then soaked in 75% alcohol 20s, wash 4 times with sterile wa...

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Abstract

The invention discloses a method for improving regeneration efficiency of wild tallow excellent strain stems. The method comprises steps of: taking a current-year stem of wild tallow excellent strain as an explant; and obtaining a regenerated robust tallow plant by going through six steps: disinfection, induction, proliferation, elongation, rooting and transplanting. Compared to the prior art, the method of the invention has the following advantages: being capable of maintaining germplasm characteristics of female plants; materials are easy to obtain and are rich, thereby implementing fast propagation of fine wild tallow individuals and variety development. The disinfection procedure is simple, disinfection time is short and less toxin is caused to the explant; the pollution rate is low, cluster buds have a high regeneration rate, the regeneration speed is fast, and the propagation coefficient is high; the method is applicable to all gene types of tallow,and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for improving the stem segment regeneration efficiency of excellent strains of Chinese tallow tree in the field. Background technique [0002] Sapium sebiferium (Sapium sebiferium), also known as wax tree and tallow tree, is a perennial deciduous tree of the genus Sapium in Euphorbiaceae. . The oil content of Chinese tallow tree seeds is as high as 55%. It is an ideal raw material for biodiesel production and is a unique economic tree species for oil in China. Accompanied by strong resistance to cold, drought, waterlogging and salt-alkali, it is an excellent landscaping tree species and has a cultivation history of 1,400 years in China. Therefore, Chinese tallow tree has important economic and ecological value whether it is used as an economic crop for oil or as a tree species for landscaping. With the accelerated development of biomass energy forestry and landscape...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 吴丽芳侯金艳刘文博穆燕陈晶李明浩毛颖基赵薇薇
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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