A semi-preparative chromatographic column fast filling device and its preparation method

Abstract

The invention belongs to the technical field of chromatographic separation, and particularly relates to a semi-preparative chromatographic column quick filling device and a preparation method thereof. The semi-preparative chromatographic column quick filling device includes a common horizontal oscillation table, a base plate, a liquid suction hose, a centrifugal pump, and a plurality of independent semi-preparative chromatographic column units, wherein each semi-preparative chromatographic column unit includes a feeding device, an oscillator ring clamp, an oscillator, a chromatographic column, a quartz spacer and a chromatographic column clamping block. Compared with the prior art, the semi-preparative chromatographic column quick filling device and the preparation method thereof, provided by the invention, have the following advantages: the filling process of a filler during oscillation and liquid suction is accelerated, so that the test time is effectively reduced, and the separation cycle of a sample is shortened; multiple chromatographic columns work simultaneously, so that the chromatographic processing amount is increased; the compactness of the chromatographic column bed is controlled jointly through oscillation frequency and time, so that the stability of the sample separation and purification effect and the test repeatability are ensured.

Description

technical field [0001] The invention belongs to the technical field of chromatographic separation, and in particular relates to a semi-preparative chromatographic column rapid filling device and a preparation method thereof. Background technique [0002] Chromatographic separation technology utilizes that different substances have different distribution coefficients in a system composed of a stationary phase and a mobile phase. When the two phases move relative to each other, these substances move together with the mobile phase and are repeatedly distributed between the two phases. So that the various substances can be separated. In the process of preparative and semi-preparative chromatographic separation, the chromatographic column is filled by the experimenter, and the process of bed compaction is usually settled by the weight of the filler or washed by the eluent. The former speed is too slow, which will occupy a large proportion of the test process. The latter can cont...

AI Technical Summary

Problems solved by technology

In the process of preparative and semi-preparative chromatographic separation, the chromatographic column is filled by the experimenter, and the process of bed compaction is usually settled by the weight of the filler or washed by the eluent. The former speed is too slow, which will occupy a large proportion of the test process. The latter can control the compactness of the bed to a certain extent, but due to the uneven distribution at the top of the chromatographic column, the middle part of the top of the bed is often lower than the edge, and the top of the bed is not on the same plane

During the liquid absorption process, the laboratory generally uses a rubber-tip dropper or ear wash ball to absorb the organic solvent on the upper end of the chromatographic column, which is inefficient and easy to stick to the skin

In the process of operation, the experimenter will not be exactly the same bed layer due to random and accidental factors every time the column is loaded. If the sample is chromatographically separated or purified under the condition of other test conditions, different results will be obtained, which reduces the test efficiency. Repeatability and Product Stability

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