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Dunaliella salina culture medium and culture method

A technology of Dunaliella salina and a culture method is applied in the field of food science to achieve the effects of facilitating synthesis, increasing synthesis amount and stabilizing culture conditions

Inactive Publication Date: 2015-10-21
中山鼎晟生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, relevant studies have shown that the optimum phosphorus source for the growth of Salina salina is phosphate, and the optimum nitrogen source is nitrate, and studies on the optimal concentration of these two inorganic salts and other elements required for the growth of Salina salina Rarely, for this reason, the present invention provides a kind of culture medium, can improve salina output especially can improve its β-carotene content

Method used

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  • Dunaliella salina culture medium and culture method
  • Dunaliella salina culture medium and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A Dunaliella salina culture medium, the prescription and proportioning of described culture medium are:

[0051] NaNO3 20mg / L,

[0052] Na3PO4 20mg / L,

[0053] K2HPO4 15mg / L,

[0054] Iron salt mother liquor 10ml / L,

[0055] 7 H2O-ZnSO4 6mg / L,

[0056] 4 H2O-MnCl2 4mg / L,

[0057] 5 H2O-CuSO4 45ug / L,

[0058] Sea salt 200 g / L,

[0059] Borax 0.03g / L,

[0060] CaCl2 0.05 mg / L,

[0061] The balance is water.

[0062] The formula and ratio of the iron salt mother liquor are: Na2-EDTA 1.0mg / L and FeCL3.6H2O 0.25mg / L.

[0063] Adopting the above-mentioned Dunaliella salina culture medium to carry out the method for cultivating salina, comprising the following steps:

[0064] (1) First prepare the Salina culture medium, and the formula and ratio of the medium are as follows:

[0065] NaNO3 20mg / L,

[0066] Na3PO4 20mg / L,

[0067] K2HPO4 15mg / L,

[0068] Iron salt mother liquor 10ml / L,

[0069] 7 H2O-ZnSO4 6mg / L,

[0070] 4 H2O-MnCl2 4mg / L,

[0071] 5 H2O-CuSO4 4...

Embodiment 2

[0081] A kind of Dunaliella salina culture medium, the prescription and the proportioning of described culture medium are:

[0082] NaNO3 60mg / L,

[0083] Na3PO4 40mg / L,

[0084] K2HPO4 20mg / L,

[0085] Iron salt mother liquor 15ml / L,

[0086] 7 H2O-ZnSO4 8mg / L,

[0087] 4 H2O-MnCl2 6mg / L,

[0088] 5 H2O-CuSO4 54ug / L,

[0089] Sea salt 250 g / L,

[0090] Borax 0.2g / L,

[0091] CaCl2 0.1 mg / L,

[0092] The balance is water.

[0093] Preferably, the composition and ratio of the iron salt mother liquor are: Na2-EDTA 1.5mg / L and FeCL3.6H2O 2mg / L.

[0094] Adopting the above-mentioned Dunaliella salina culture medium to carry out the method for cultivating salina, comprising the following steps:

[0095] (1) First prepare the Salina culture medium, and the formula and ratio of the medium are as follows:

[0096] NaNO3 60mg / L,

[0097] Na3PO4 40mg / L,

[0098] K2HPO4 20mg / L,

[0099] Iron salt mother liquor 15ml / L,

[0100] 7 H2O-ZnSO4 8mg / L,

[0101] 4 H2O-MnCl2 6mg / L...

Embodiment 3

[0111] A kind of Dunaliella salina culture medium, the prescription and the proportioning of described culture medium are:

[0112] NaNO3 40mg / L,

[0113] Na3PO4 30mg / L,

[0114] K2HPO4 18mg / L,

[0115] Iron salt mother liquor 13ml / L,

[0116] 7 H2O-ZnSO4 7mg / L,

[0117] 4 H2O-MnCl2 5mg / L,

[0118] 5 H2O-CuSO4 50ug / L,

[0119] Sea salt 230g / L,

[0120] Borax 0.1g / L,

[0121] CaCl2 0.08mg / L,

[0122] The balance is water.

[0123] Preferably, the composition and ratio of the iron salt mother liquor are: Na2-EDTA 1.3 mg / L and FeCL3.6H2O 1 mg / L.

[0124] Adopting the above-mentioned Dunaliella salina culture medium to carry out the method for cultivating salina, comprising the following steps:

[0125] (1) First prepare the Salina culture medium, and the formula and ratio of the medium are as follows:

[0126] NaNO3 40mg / L,

[0127] Na3PO4 30mg / L,

[0128]K2HPO4 18mg / L,

[0129] Iron salt mother liquor 13ml / L,

[0130] 7 H2O-ZnSO4 7mg / L,

[0131] 4 H2O-MnCl2 5mg / L...

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Abstract

The invention relates to a dunaliella salina culture medium. The culture medium per litter comprises 20-60 mg of NaNO3, 20-40 mg of Na3PO4, 15-20 mg of K2HPO4, 10-15 ml of iron salt stock solution, 6-8 mg of 7H2O-ZnSO4, 4-6 mg of 4H2O-MnCl2, 45-54 ug of 5H2O-CuSO4, 200-250 g of sea salt, 0.03-0.2 g of borax, 0.05-0.1 mg of CaCl2 and the balance of water. By adopting the culture medium for culturing dunaliella salina, cell proliferation of the dunaliella salina can be promoted, the content of beta-carotenes in single dunaliella salina cells can reach the maximum value, and meanwhile the synthesis quantity of dunaliella salina protein can be obviously increased.

Description

technical field [0001] The invention belongs to the field of food science and technology, and in particular relates to a Dunaliella salina culture medium and a cultivation method. Background technique [0002] Salina has strong adaptability to salinity, and can grow in various salinity conditions close to fresh water to saturated salt water, and is widely distributed in high-salinity areas such as salt lakes and oceans. Salina is the only species without a cell wall, and it belongs to a class of lower eukaryotes. Salina is rich in polysaccharides, trace elements, proteins, highly unsaturated fatty acids and other nutrients. At present, it has been used in many fields. For example, it can be used as bait for larvae such as fish and shrimp, and it can also be used as an important antioxidant and moisturizing ingredient in the field of skin care cosmetics. [0003] The molecular formula of β-carotene is C 40 H 56 , and the relative molecular weight is 536.88. It is composed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 郭狄
Owner 中山鼎晟生物科技有限公司
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