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Multifunctional decontaminating column used for detecting alternaria spp toxin and use method thereof

A multifunctional purification column and Alternaria technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of poor detection and purification effect of Alternaria toxin, complicated reagents, and inability to detect, and achieves convenient and economical pretreatment. Time and cost, effect of reducing consumption

Active Publication Date: 2015-10-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to solve the problem that the existing solid phase extraction column technology has poor detection and purification effect on Alternaria toxin, low recovery rate, and relatively complicated reagents, which cannot accurately detect various fruits, vegetables, grains and food samples. , and then propose a multi-functional purification column for Alternaria toxin detection and its application method

Method used

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  • Multifunctional decontaminating column used for detecting alternaria spp toxin and use method thereof
  • Multifunctional decontaminating column used for detecting alternaria spp toxin and use method thereof
  • Multifunctional decontaminating column used for detecting alternaria spp toxin and use method thereof

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Effect test

Embodiment 1

[0041] Embodiment 1: raw material and preparation of multifunctional purification column

[0042] 1. Raw materials:

[0043] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0044] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 μm;

[0045] (3) Reverse-phase chromatography filler: 200 μL particle size of 40-60 μm reverse-phase chromatography filler HLB;

[0046] (4) Cation exchange chromatography filler: 200 μL of cation exchange chromatography filler MCX with a particle size of 40-60 μm.

[0047] 2. Assembly method (see attached figure 1 )

[0048]to polypropylene or glass column tubes (attached figure 1 In step 1), first fill a piece of sieve plate (attached figure 1 6) in , add 200μL cation exchange chromatography filler (attached figure 1 5) in, and then fill in the second sieve plate (attached figure 1 4) in , add 200 μL of reversed-phase chromatography filler (attached fig...

Embodiment 2

[0049] Embodiment 2: raw material and preparation of multifunctional purification column

[0050] 1. Raw materials

[0051] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0052] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 microns;

[0053] (3) Reverse-phase chromatography filler: 600 μL particle size of 40-60 μm reverse-phase chromatography filler HLB;

[0054] (4) Cation exchange chromatography filler: 200 μL of cation exchange chromatography filler MCX with a particle size of 40-60 μm.

[0055] 2. Assembly method

[0056] Fill a polypropylene or glass column tube with a sieve first, add 200 μL of cation exchange chromatography packing material, then fill in the second sieve plate, add 600 μL reverse phase chromatography packing material, then fill in the third sieve plate, and press tightly.

Embodiment 3

[0057] Embodiment 3: raw material and preparation of multifunctional purification column

[0058] 1. Raw materials

[0059] (1) Column tube: 6mL polypropylene or glass column tube with an inner diameter of 12.7mm;

[0060] (2) Sieve plate: polyethylene or polytetrafluoroethylene sieve plate with a pore size of 10-30 microns;

[0061] (3) Reverse-phase chromatographic filler: 600 μL particle size of 40-60 μm reverse-phase chromatographic filler;

[0062] (4) Cation exchange chromatography filler: 600 μL of cation exchange chromatography filler with a particle size of 40-60 μm;

[0063] 2. Assembly method

[0064] Fill a polypropylene or glass column tube with a sieve first, add 600 μL of cation exchange chromatography packing material, then fill in the second sieve plate, add 600 μL reverse phase chromatography packing material, then fill in the third sieve plate, and press it tightly.

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Abstract

The invention relates to a multifunctional decontaminating column used for detecting alternaria spp toxin and a use method thereof. The multifunctional decontaminating column comprises reversed phase chromatographic packing and cation-exchange chromatography packing. The multifunctional decontaminating column can be suitable for various fruit samples, various vegetable samples, various grain samples, various feed samples and various food samples.

Description

technical field [0001] The invention relates to a method for making and using a multifunctional purification column, in particular to a multifunctional purification column for detecting Alternaria toxin and a method for using the same. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. Their pollution in crop products, food and feed is becoming more and more serious, causing great harm to human and animal health. Among them, Alternaria toxins produced by Alternaria spp are a type of mycotoxins with a high incidence worldwide. It widely pollutes fruits and vegetables, wheat, oilseeds and other agricultural products and their primary processed products, among which the detection rate is higher in fruits and vegetables and their products. This may be due to the fact that Alternaria can grow and reproduce at low temperature and produce toxins during the storage of fruits and vegetables. In addition, Alternaria can also cause internal rot of s...

Claims

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Application Information

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IPC IPC(8): G01N30/60
Inventor 王蒙冯晓元姜楠韦迪哲王纪华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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