Application of compound p7c3‑a20 in the preparation of drugs for treating cerebral ischemic diseases

A technology of P7C3-A20 and compounds, applied in the field of compounds, can solve the problem that P7C3-A20 has no protective effect on cerebral ischemic injury, and achieve the effects of reducing the size of cerebral infarction, broad application prospects, and improving survival rate

Active Publication Date: 2018-04-17
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no report on the protective effect of P7C3-A20 on cerebral ischemic injury

Method used

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  • Application of compound p7c3‑a20 in the preparation of drugs for treating cerebral ischemic diseases
  • Application of compound p7c3‑a20 in the preparation of drugs for treating cerebral ischemic diseases
  • Application of compound p7c3‑a20 in the preparation of drugs for treating cerebral ischemic diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Method

[0020] 1. Culture of mouse cerebral cortex neurons

[0021] (1) Pregnant mice were taken, E15-18 days, sacrificed by dislocation of the cervical spine, wiped the abdomen with 75% alcohol, laparotomy, and excised the uterus.

[0022] (2) Place the uterus in a petri dish containing double-antibody sterile deionized water, and transfer it to the cell room.

[0023] (3) Quickly transfer the uterus to ice-cold high-glucose DMEM, tear the uterus from the uterine horns with ophthalmic scissors, take out the fetal mouse, decapitate it with scissors, peel it off carefully, and take the brain; separate the cerebral cortex under a microscope and carefully peel it off meninges and blood vessels.

[0024] (4) Transfer the brain tissue into fresh ice-cold high-glucose DMEM, rinse twice, transfer to a 1.5mL EP tube, place it on ice, and cut the brain tissue as much as possible with curved scissors.

[0025] (6) Transfer the shredded brain tissue to a small Erlenmeyer fl...

Embodiment 2

[0040] 1. Method

[0041] Rats (Sprague-Dawley rats, weighing about 250 g, Shanghai Slack Experimental Animal Co., Ltd.) were used to prepare a permanent cerebral ischemic injury model. The process is as follows: rats were anesthetized, the skull was opened, and the middle cerebral artery was electrocoagulated directly under visual observation, resulting in blockage of the left middle cerebral artery. Thirty minutes later, rats were given intraperitoneal injection of P7C3-A20 (30 mg / kg). The skull was reset and the skin was sutured, and the rat model of permanent cerebral ischemia caused by occlusion of the middle cerebral artery was successfully prepared. Twenty-four hours after middle cerebral artery occlusion, the cerebral infarct area was detected. The volume of cerebral infarction was detected by 2,3,5-triphenyltetrazolium chloride (purchased from Amersco) staining method, that is, TTC staining method.

[0042] 2. Results

[0043] The results are shown in Table 1. It ...

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Abstract

The present invention relates to the application of compound P7C3-A20 in the preparation of medicines for treating cerebral ischemic diseases. The present invention confirms through cell experiments that P7C3‑A20 can improve the survival rate of mouse cerebral cortex neurons cultured in vitro in the model of hypoxia and glucose deficiency. These results indicate that P7C3‑A20 has a protective effect on cerebral ischemia, can prevent and treat brain damage caused by cerebral ischemia, and has broad application prospects in the preparation of drugs related to cerebral ischemic injury and / or cerebral infarction.

Description

technical field [0001] The present invention relates to a new application of the compound, in particular to the application of the compound P7C3-A20 in the preparation of a drug for treating cerebral ischemic diseases. Background technique [0002] P7C3-A20 is one of the P7C3 series compounds, the CAS number is 1235481-90-9, and the chemical structural formula is: [0003] [0004] It is a chloropropylcarbazole compound with high oral bioavailability, crosses the blood-brain barrier, and exhibits no toxicity at both effective and high doses. Foreign reports have found that P7C3 compounds have protective effects in depression, senile diseases such as Parkinson's (PD), amyotrophic lateral sclerosis (ALS), cognitive degenerative diseases, retinal degeneration and other diseases . At present, there is no report on the protective effect of P7C3-A20 on cerebral ischemic injury. Contents of the invention [0005] The purpose of the present invention is to provide a new appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/403A61P9/10
Inventor 缪朝玉王培张赛龙王淑娜徐添颖管云枫
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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