Microbial remediation method capable of mineralizing heavy metal ions based on glyphosate degradation
A heavy metal ion and glyphosate technology, applied in the field of microbiology, can solve problems such as high energy consumption, complicated operation, and secondary pollution, and achieve the effects of stable mineral properties, slowing down the greenhouse effect, and strong durability
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[0036] (1) Weigh 6g of peptone, 4g of beef extract, 5g of NaCl, 18g of agar, MgSO 4 Dissolve 0.5g in 1000ml of deionized water, and control the pH to 7, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate Bacillus subtilis into the cooling medium solution, and culture it with shaking at 37°C, the shaking frequency is 170r / min, culture time 24h; centrifuge the cultivated bacterial liquid at high speed for 10min, the speed of the centrifuge is 8000rpm, the temperature is 4°C, remove the nutrients in the upper medium, add 100mL of deionized water to make concentrated bacterial liquid.
[0037] (2) Weigh 18g of agar and dissolve it in 1000ml of wort at 5°C, sterilize at 125°C for 30 minutes, take it out and wait for cooling; inoculate the yeast into the cooling medium solution, and culture at 30°C with shaking at a frequency of 170r / min, culture time 24h; Centrifuge the cultured bacterial solution at high speed for 10min, the speed of the centrifuge is...
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