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Total bile acid measuring kit

A technology of total bile acid and kit, applied in the field of biochemical detection, can solve problems such as poor stability

Active Publication Date: 2015-10-28
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the disadvantages of poor stability of the existing total bile acid assay reagents, and to provide a continuously stable solution under the condition of opening the cover at 2-8°C and under the condition of airtight and light-proof storage at 2-8°C. Total Bile Acid Assay Kit

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Reagents A were prepared according to the contents of the components listed in Table 1, and the components were mixed to obtain a uniform stable phase to obtain reagents A1-21 respectively.

[0062] Table 1

[0063]

[0064]

[0065] In reagent A1-21, the specific selection of each component is as follows:

[0066] In reagents A1-17 and 21, the surfactant is polyoxyethylene lauryl ether (EMULGEN109P), the buffer A is 2-N-morphine ethanesulfonic acid buffer, and the stabilizer A is MgCl 2 It is made by mixing with EDTA.2Na according to the mass ratio of 1:1.

[0067]In reagent A18, the surfactant is polyethylene glycol p-isooctyl phenyl ether (Triton TX-100), the buffer A is 2-N-morphine ethanesulfonic acid buffer, and the stabilizer A is MgCl 2 It is made by mixing with EDTA.2Na according to the mass ratio of 1:1.

[0068] In reagent A19, the surfactant is polyethylene glycol p-isooctyl phenyl ether (Triton TX-100), the buffer A is glycine buffer, and the stabi...

Embodiment 2

[0076] Reagent B was prepared according to the component contents listed in Table 2 to obtain reagents B1-21 respectively.

[0077] The preparation method is as follows: sequentially mixing buffer solution, surfactant B, stabilizer B, reduced coenzyme I and 3α-HSD to obtain a uniform stable phase.

[0078] Table 2

[0079]

[0080] In reagent B 1-24, the specific selection of each component is as follows:

[0081] In reagent B 1-21, buffer B is N-tris(hydroxymethyl)-3-aminopropanesulfonic acid buffer at pH 8.5, stabilizer B is sodium adenosine-3',5'-cyclic monophosphate salt; the surfactant is polyoxyethylene lauryl ether (EMULGEN109P).

[0082] In reagent B 22, buffer B is 3-(cyclohexylamine)-1-propanesulfonic acid (CAPS), stabilizer B is adenosine-3',5'-cyclic monophosphate sodium salt; surfactant is poly Oxyethylene lauryl ether (EMULGEN109P).

[0083] In reagent B 23, buffer solution B is N-tris(hydroxymethyl)-3-aminopropanesulfonic acid at pH 8.5, stabilizer B is s...

Embodiment 3

[0092] Example 3 is used to illustrate the preparation of the calibrator. Dissolve the pure product sodium glycocholate and the stabilizer mannitol in the matrix 2-N-morphine ethanesulfonic acid buffer and bovine serum albumin, and mix uniformly to obtain the calibrator, wherein , the concentration of sodium glycocholate is 50 μmol / L, the concentration of mannitol is 20g / L, the concentration of bovine serum albumin is 10% by mass volume percentage, and the concentration of 2-N-morphine ethanesulfonic acid buffer The concentration is 100mmol / L.

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Abstract

The invention provides a total bile acid measuring kit. The kit comprises a reagent A and a reagent B, wherein the reagent A comprises Thio-NAD, a buffer solution A, a surface active agent and a stabilizing agent A; the reagent B comprises 3 alpha-HSD, reduced coenzyme I, a surface active agent, a buffer solution B and a stabilizing agent B. Through the technical scheme, when the total bile acid measuring kit is in an uncovered state for use at the temperature ranging from 2 DEG C to 8 DEG C, the stabilization time can reach 20-30 days, and the stabilization time in the airtight and light shielding storage process at the temperature ranging from 2 DEG C to 8 DEG C can reach 10-15 months.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a total bile acid assay kit. Background technique [0002] In 1974, the Norwegian Comyn Company successfully produced the first generation of bile acid enzymatic reagents using purified 3α-hydroxysteroid dehydrogenase (3α-HSD), making the determination of bile acids truly enter the clinical field. Since then, Norway's Kemin and Japan's Daiichi Chemical have improved the reagent components of the first-generation reagent, and developed the second-generation bile acid determination reagent by using the starter reagent. After that, Japan's Daiichi Chemical purified the 3 -Oxy-5β-steroids- △ 4 dehydrogenase ( △ 4DH) was coupled with 3α-HSD to develop a third-generation bile acid assay kit. [0003] Chinese patent CN1281983 discloses a serum total bile acid assay reagent, which is a reagent I composed of a buffer containing nitroblue tetrazolium, oxidized coenzyme I, diaphorase...

Claims

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Application Information

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IPC IPC(8): C12Q1/32G01N21/31
Inventor 岳彩琴
Owner BIOSINO BIO TECH & SCI
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