Automatic sample processing device and automatic workstation for original microbial oil and gas and hydrate exploration technology
A sample processing and hydrate technology, which is applied in the direction of biological material pretreatment, biochemical cleaning equipment, enzymology/microbiology equipment, etc., can solve the problems of many data influencing factors, undiscoverable, poor data reliability, etc., and achieve data reliability Good performance, ensure accuracy, realize the effect of automation
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Embodiment 1
[0038] This embodiment provides an automatic sample processing device for original state microbial oil gas and hydrate exploration technology, including automatic liquid processing device and nucleic acid extraction equipment;
[0039] The automatic liquid processing device automatically adds samples, prepares and processes samples and reaction reagents, and through the integration of various terminal analysis and detection equipment, the entire experimental process is fully automated in an unmanned environment and results are given. Preferably, the automatic liquid processing device is composed of four parts: an operating platform, a mechanical arm, a pipetting channel, and a carrier and auxiliary equipment for experimental supplies; several positioning tracks (T) are arranged on the operating platform, It is used to fix the carrier for various experimental supplies. The operating platform is the frame of the entire workstation, which is a semi-closed structure, and there is ...
Embodiment 2
[0048] Based on the automatic sample processing device for the original microbial oil gas and hydrate exploration technology described in the present invention, this embodiment provides an automatic workstation for the original microbial oil gas and hydrate exploration technology. The workstation includes the original microbial oil gas and hydrate In addition to the automatic processing device for geophysical prospecting technology, it also includes a laminar flow hood and a PCR reaction system configuration system.
[0049] An ultra-clean laminar flow hood is set above the automatic liquid processing device and / or nucleic acid extraction equipment to ensure that the experimental process works in a sterile environment, and a ventilation pipeline can also be connected to discharge or recycle harmful substances generated in the experiment. Such a setting can not only protect the health and safety of operators, but also ensure the safety of samples and experimental procedures.
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Embodiment 4
[0114] Embodiment 4 Automated Workstation Work Performance Test of the present invention
[0115] 1. Ability to handle samples
[0116] Summarize from the detection system operation record of the present invention, the start time of processing 96 marine sediment parallel samples is 11:06:26 seconds, and the end time is 12:46:03 seconds, about 1 hour and 40 minutes (i.e. 100 minutes) minutes); the detection system of the present invention runs for 8 hours per day, then the number of samples that can be processed every day=(8 hours×60 minutes / hour)×96 / 100 minutes]=461; the annual running time is calculated by 300 days, then this The invention detection system can handle 138,300 samples per year.
[0117] 2. The effect of extracting nucleic acid
[0118] From the 96 nucleic acid samples obtained above, 9 samples were arbitrarily selected for PCR amplification, and its electrophoresis was as follows: Figure 4 shown. In order to compare the effect of nucleic acid extraction, t...
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