Ultra-low temperature preservation method of eustoma pollen
A technology for ultra-low temperature preservation and eustoma, which is applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problems of short lifespan of eustoma pollen, achieve the effect of simple and easy method, and ensure the viability of pollen
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Embodiment 1
[0018] Take eustoma 'Sherry champagne' as an example
[0019] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities, and wrap the sieved pollen with sulfuric acid paper. The mass ratio of desiccant to pollen is 300: 1. Put it in an airtight glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 3 hours, so that the water content of the pollen is 6%;
[0020] (2) Cryopreservation method: put the pollen from step (1) into a cryopreservation tube and refrigerate at 4°C for 3 hours, then transfer to -20°C for 5 hours, put it in liquid nitroge...
Embodiment 2
[0027] Take eustoma "Mythical purple fringe" as an example
[0028] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities. The pollen after the sieve is wrapped in sulfuric acid paper, and the mass ratio of desiccant to pollen is 250: 1. Put it in a closed glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 5 hours, so that the water content of the pollen is 7%;
[0029] (2) Cryopreservation method: Put the pollen from step (1) into a cryopreservation tube and refrigerate at 2°C for 2 hours, then transfer to -15°C for 3 hours, put it i...
Embodiment 3
[0036] Eustoma 'Lusita' as an example
[0037] (1) Pollen collection and drying: Select healthy Eustoma plants with normal development and no damage from diseases and insect pests, collect Eustoma flowers 1 to 2 days after flowering, take out the anthers, spread the anthers evenly on waxed paper, and place them at room temperature , use a 600-watt incandescent lamp to illuminate at a distance of 1 meter. After the anthers are loosened, sieve them through a 120-mesh sieve to remove impurities, and wrap the sieved pollen with sulfuric acid paper. The mass ratio of desiccant to pollen is 350: 1. Put it in a closed glass container together with the desiccant color-changing silica gel, and dry it at room temperature for 2 hours, so that the water content of the pollen is 4%;
[0038] (2) Cryopreservation method: put the pollen from step (1) into a cryopreservation tube and refrigerate at 6°C for 5 hours, then transfer to -25°C for 6 hours, put it in liquid nitrogen and store it for...
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