Rosa canina fruit enzyme health beverage production method
A health-care beverage and a production method technology, applied in the field of bioengineering, can solve problems such as inability to retain enzyme activity for a long time, inability to ensure no pathogenic bacteria, long fermentation time, etc., and achieve the effects of inhibiting the growth of miscellaneous bacteria, retaining enzyme activity, and maintaining stable quality.
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Embodiment 1
[0023] A method for fermenting rose hip enzymes, comprising the following steps:
[0024] (1) Choose mature rose hips with fresh color and high natural enzyme content as the main raw material for fermentation.
[0025] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 30% of the volume, add 5% of the total liquid volume % of the raw honey is the fermentation stock solution for later use.
[0026] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0027] (4) According to the inoculation ratio of 5%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.
[0028] (...
Embodiment 2
[0030] A method for fermenting rose hip enzymes, comprising the following steps:
[0031] (1) Choose mature rose hips with fresh color and high natural enzyme content as the main raw material for fermentation.
[0032] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 40% of the volume, add 10% of the total liquid volume % of the raw honey is the fermentation stock solution for later use.
[0033] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0034] (4) According to the inoculation ratio of 4%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.
[0035] ...
Embodiment 3
[0037] A method for fermenting rose hip enzymes, comprising the following steps:
[0038] (1) Choose mature rose hips with fresh color and high natural enzyme content as the main raw material for fermentation.
[0039] (2) Wash twice with pure water, and finally soak in 0.9% NaCl solution, sterilize at low temperature, then drain, put into a container cleaned with pure water, accounting for about 50% of the volume, add 15% of the total liquid volume % of the raw honey is the fermentation stock solution for later use.
[0040] (3) preparing LB medium, activating the original preservation strain, and cultivating the fermented bacterial liquid obtained for about 48 hours for subsequent use;
[0041] (4) According to the inoculation ratio of 3%, the nectar-derived bacteria, Lactobacillus casei and Lactobacillus plantarum were respectively taken and inserted into the fermentation stock solution, and fermented for 7 days, with a pH value of 7.0 and a temperature of 25°C.
[0042] ...
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