Method for extracting and separating NEO-Z (4,8-dimethylenenaphthalene-1,5(4H,8H)-dione) from pericarpium juglans
A technology of methylnaphthene and green bark, which is applied in the field of extraction and separation of 4,8-dimethylnaphthene-1,5-dione, which can solve the problems of environmental pollution, inability to be directly used as agricultural fertilizers, inedibility, etc.
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[0029] The preparation method of compound of the present invention is as follows:
[0030] (1) Take 1kg green walnut peel and put it into water, the weight ratio of green walnut peel and water is 1:2-1:10;
[0031] (2) soaking and extracting at room temperature for 20-50 minutes; soaking can be assisted by ultrasonic waves or microwaves, so that the soaking time will be shorter than ordinary soaking time.
[0032] (3) Move the leached water phase into a round bottom flask, place it on a rotary evaporator, and evaporate under reduced pressure until the aqueous solution in the round bottom flask is almost colorless; during vacuum distillation, control the water bath temperature to 50°C-70°C , the vacuum degree is 0.06-0.08MPa. At this time, the yellow transparent aqueous solution in the receiving bottle is the aqueous solution containing the compound of the present invention.
[0033] (4) Take out the aqueous solution in the receiving bottle, add petroleum ether, and repeatedl...
Embodiment 1
[0038] (1) Put 1kg of fresh walnut green peel into 2L of water;
[0039] (2) Soak at room temperature for 30 minutes;
[0040] (3) Move the water phase after leaching into a round-bottomed flask, place it on a rotary evaporator and distill under reduced pressure until the aqueous solution in the round-bottomed flask is almost colorless, and it takes 30 minutes; The degree of vacuum is 0.07MPa. At this time, the yellow transparent aqueous solution in the receiving bottle is the aqueous solution containing the compound of the present invention.
[0041] (4) Take out the aqueous solution in the receiving bottle, add 1L of petroleum ether in three times for extraction, take the petroleum ether phase, and discard the water phase.
[0042] (5) Place the extracted petroleum ether phase on a rotary evaporator, and distill under reduced pressure under the condition that the temperature of the water bath does not exceed 30°C to obtain a yellow crude extract, weighing about 500 mg; the...
Embodiment 2
[0045] (1) Add 200g of fresh walnut green peel to 1L of water;
[0046] (2) Soak at room temperature for 20 minutes;
[0047] (3) Move the leached water phase into a round-bottomed flask, place it on a rotary evaporator, and evaporate it under reduced pressure for 20 minutes. At this time, the aqueous solution in the round-bottomed flask is almost colorless; during vacuum distillation, the temperature of the water bath is controlled to be 70 ° C. , the vacuum degree is 0.065MPa. At this time, the yellow transparent aqueous solution in the receiving bottle is the aqueous solution containing the compound of the present invention.
[0048] (4) Take out the aqueous solution in the receiving bottle, add 0.5L petroleum ether for extraction three times, take the petroleum ether phase, and discard the water phase.
[0049] (5) Place the extracted petroleum ether phase on a rotary evaporator, and distill under reduced pressure under the condition that the temperature of the water bat...
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