Application of phillyrin in preparation of digestants

A technology for forsythin and digestion aid, which is applied in the application field of forsythin in the preparation of digestive aid drugs, and can solve the problems of affecting curative effect, high price of enzyme preparations, and decreased activity

Active Publication Date: 2015-11-18
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the price of enzyme preparations is generally more expensive, and the activity decreases during the extraction and production process, which affects the curative effect

Method used

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  • Application of phillyrin in preparation of digestants
  • Application of phillyrin in preparation of digestants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 100 μL of PL solution (0.5 mg / mL) and place it in a 1.5 mL centrifuge tube, add 20 μL of 5 mg / mL forsythin solution, dilute to 900 μL with pH=7.5 Tris-HCl buffer, mix well, and incubate at 37°C for 15 min. Then add 100 μL of pNPB solution (10 mmol / L), shake well, and quickly transfer to a 96-well microplate. Measure the absorbance value at 400nm with a microplate reader, read the number every 3 minutes, and record the data within 15 minutes. Take time as the abscissa and the absorbance value as the ordinate, and calculate the change rate K of the absorbance value with time. The inhibition rate was calculated according to the following formula:

[0016] PL inhibition rate (%) = (K 正常组 -K 实验组 ) / K 正常组 ×100%

[0017] In the reaction system, no enzyme was used as the blank control, no forsythin was used as the normal group, and orlistat was used as the positive control, and each experiment was repeated 3 times.

[0018] Experimental results: when 20 μL of 5 mg / mL ...

Embodiment 2

[0020] Adding 20 μL of 20 mg / mL forsythin solution, that is, when the final concentration is 400 μg / mL, the activity of pancreatic lipase can be increased to 134.11%. Other conditions are with embodiment 1.

Embodiment 3

[0022] Adding 20 μL of 40 mg / mL forsythin solution, that is, when the final concentration is 800 μg / mL, the activity of pancreatic lipase can be increased to 146.43%. Other conditions are with embodiment 1.

[0023] The summary results of each embodiment are as figure 1 shown.

[0024] 2. Mechanism experiment of forsythin to promote pancreatic lipase activity:

[0025] Forsythin solutions with a concentration approximately equal to the IC50 value were prepared respectively. With no forsythin as the control group and forsythin as the experimental group, the enzyme reaction rate was measured when the pNP concentration was 0.25, 0.50, 0.75, 1.00, 1.25 mmol / L respectively. According to the Lineweaver-Burk double reciprocal plotting method. The type of inhibition was judged according to the position of the intersection of two straight lines in the control group and the experimental group.

[0026] Experimental results such as figure 2 As shown, it is proved that forsythin ha...

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Abstract

The invention provides an application of phillyrin in preparation of digestants, namely an application of phillyrin in preparation of drugs promoting pancreatic lipase activity. Phillyrin has effect of promoting pancreatic lipase activity to quicken lipodieresis of dietary fat, improve absorption of fat and lipidic solubility nutriment and reduce malnutrition symptom. According to experiment in vivo, content of pancreatic lipase activity is increased to 146. 43% after 800 mu m g/mL of phillyrin solution is added. As proved by mechanism and experiment of promoting pancreatic lipase activity by phillyrin, phillyrin has competitive promotion function to pancreatic lipase activity.

Description

technical field [0001] The invention relates to a new application of forsythin, in particular to the application of forsythin in preparing digestive medicine. Background technique [0002] At present, dyspepsia patients generally exist, one includes animal or human infants and elderly patients with dyspepsia [Zhao Gaiting et al. (2004). Nursing Research. 18 (9), 842-843; Wang Cuizhang et al. (1987). Foreign Medicine (Digestive Diseases Volume). (04); Zhao Na (2010). Feed Research. (10): 16-18], the second is due to chronic pancreatitis, pancreatic malignant tumors, pancreatic cystic fibrosis, primary Patients with pancreatic atrophy, pancreatectomy, and pancreatic lipase inactivation [Gao Honggang (2013). Practical Clinical Medicine. 14(8): 126-128; Zhang Zhihong (2005). Pancreatology. Wang Yuanben et al. (2002). World Chinese Journal of Digestion. 10(12): 1415-1418]. These patients have various etiologies, but one of them is mainly due to insufficient secretion or low act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P1/14A23L1/29A23L33/00
Inventor 陈廷贵栗亚云张立伟席小莉
Owner SHANXI UNIV
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