Preparation method of channel catfish Edwardsiellosis vaccines

A channel catfish and vaccine technology, applied in the directions of antibacterial drugs, bacterial antigen components, etc., can solve the problems of insignificant effect and high cost of channel catfish Edwardsiosis, and achieve the effects of improving immunity, remarkable control effect and convenient operation.

Inactive Publication Date: 2015-11-18
霍邱县绿波水产科技服务有限公司
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  • Summary
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  • Claims
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AI Technical Summary

Problems solved by technology

[0003] In recent years, domestic and imported drugs have no obvious curative effect on channel catfish Edwards' disease or the cost is too high, so the effect is not obvious
It can only be prevented by immunization, and this vaccine is still blank

Method used

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  • Preparation method of channel catfish Edwardsiellosis vaccines
  • Preparation method of channel catfish Edwardsiellosis vaccines
  • Preparation method of channel catfish Edwardsiellosis vaccines

Examples

Experimental program
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Embodiment Construction

[0031] The preparation method of channel catfish Edwardsiosis vaccine, concrete operation steps are as follows:

[0032] 1. Extract strains: isolate the pathogenic bacteria Edwardsiella from the liver, kidney, blood, and ascites of channel catfish with obvious symptoms of Edwardsiella, and immediately store them in a refrigerator at 4-8°C;

[0033] 2. Infection: Infect the isolated pathogenic bacteria with 1-2×10 9 Inoculate the body of healthy channel catfish at a concentration of 1 / 1. The infection test is carried out indoors to check whether it is virulent, and it is used as a vaccine material if it is proved to be virulent;

[0034] 3. Material collection: take the above-mentioned diseased channel catfish tissue material, weigh it, add 0.85% physiological to each gram tissue material and store it in a low-temperature refrigerator at -30°C to -40°C, or put it in 50% phosphate-buffered glycerol, and put it in 4- Store in a refrigerator at 8°C (be sure to rinse 3 times with ...

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Abstract

The invention provides a preparation method of channel catfish Edwardsiellosis vaccines. The preparation method includes the steps of strain extraction, infection, material acquiring, suspension preparation, enlarged cultivation, counting, inactivation, synthesizing, diluting, split charging and inspection. The channel catfish Edwardsiellosis vaccines are prepared by adding a proper amount of double-resistant and antiseptic agent into Edwardsiella. The preparation method is low in cost and convenient in operation, immunity of channel catfishes can be improved effectively, and channel catfish Edwardsiellosis is prevented.

Description

technical field [0001] The invention relates to the field of aquaculture disease prevention and control, in particular to a method for preparing a channel catfish Edwards disease vaccine. Background technique [0002] Channel catfish (Ictalurus Punctatus (Rafinesque), also known as Channel Catfish), belongs to Siluriformes (Siluriformes) and Ictaluridae (Ictaluridae) fish. It has miscellaneous feeding habits, fast growth, wide adaptability, easy feeding, easy catch and good meat quality. It is a world-renowned breeding species and fishing object for its superiority and other advantages. Channel catfish has a high meat content and is rich in nutrients. According to measurements, the meat content rate is 75.71%; crude protein accounts for 19.42% in muscle, fat accounts for 1.01%, and moisture Accounted for 77.58%, ash accounted for 1.12%, carbohydrates accounted for 0.87%; muscle contains 18 kinds of amino acids, accounting for 18.72% of the total muscle, of which the essentia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P31/04
Inventor 李进村许军曹明远刘其芝王应琴
Owner 霍邱县绿波水产科技服务有限公司
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