Preparation method of channel catfish Edwardsiellosis vaccines
A channel catfish and vaccine technology, applied in the directions of antibacterial drugs, bacterial antigen components, etc., can solve the problems of insignificant effect and high cost of channel catfish Edwardsiosis, and achieve the effects of improving immunity, remarkable control effect and convenient operation.
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[0031] The preparation method of channel catfish Edwardsiosis vaccine, concrete operation steps are as follows:
[0032] 1. Extract strains: isolate the pathogenic bacteria Edwardsiella from the liver, kidney, blood, and ascites of channel catfish with obvious symptoms of Edwardsiella, and immediately store them in a refrigerator at 4-8°C;
[0033] 2. Infection: Infect the isolated pathogenic bacteria with 1-2×10 9 Inoculate the body of healthy channel catfish at a concentration of 1 / 1. The infection test is carried out indoors to check whether it is virulent, and it is used as a vaccine material if it is proved to be virulent;
[0034] 3. Material collection: take the above-mentioned diseased channel catfish tissue material, weigh it, add 0.85% physiological to each gram tissue material and store it in a low-temperature refrigerator at -30°C to -40°C, or put it in 50% phosphate-buffered glycerol, and put it in 4- Store in a refrigerator at 8°C (be sure to rinse 3 times with ...
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