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Method for synthesizing oleic acid/sodium oleate vesica under alternating electric field

An alternating electric field and sodium oleate technology, applied in cell biology, can solve the problems of small vesicles, expensive, difficult to observe, etc., and achieve the effect of fast response, low cost, and uniform size

Active Publication Date: 2015-11-18
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the existing method for synthesizing vesicles or the use of relatively expensive phospholipids as raw materials, which limits its development as a drug carrier, or the problem that the vesicles synthesized by oleic acid are small and difficult to observe under an optical microscope , while providing a method for synthesizing oleic acid / sodium oleate vesicles under an alternating electric field

Method used

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  • Method for synthesizing oleic acid/sodium oleate vesica under alternating electric field
  • Method for synthesizing oleic acid/sodium oleate vesica under alternating electric field
  • Method for synthesizing oleic acid/sodium oleate vesica under alternating electric field

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specific Embodiment approach 1

[0017] Specific implementation mode one: combine figure 1 Describe this embodiment in detail, a method for synthesizing oleic acid / sodium oleate vesicles under an alternating electric field described in this embodiment is specifically carried out according to the following steps:

[0018] 1. Prepare methanol solution of oleic acid / sodium oleate: dissolve oleic acid and sodium oleate in methanol, and ultrasonicate for 5-20 minutes to obtain oleic acid / sodium oleate methanol solution;

[0019] The concentration of oleic acid in the described oleic acid / sodium oleate methanol solution is 0.005mol / L~0.02mol / L; the concentration of sodium oleate in the described oleic acid / sodium oleate methanol solution is 0.005mol / L~0.02mol / L; And the molar ratio of oleic acid and sodium oleate in the described oleic acid / sodium oleate methanol solution is 1:1;

[0020] 2. Pretreatment of ITO electrodes: Clean the two ITO electrodes ultrasonically with distilled water for 5 minutes to 15 minute...

specific Embodiment approach 2

[0027] Specific embodiment two: this embodiment is different from specific embodiment one: the concentration of oleic acid in the oleic acid / sodium oleate methanol solution described in step one is 0.015mol / L; Described oleic acid / sodium oleate The concentration of sodium oleate in methanol solution is 0.015mol / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that in step 2, the two ITO electrodes are ultrasonically cleaned with distilled water for 5 minutes, and then ultrasonically cleaned with absolute ethanol for 5 minutes. Others are the same as in the first or second embodiment.

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Abstract

The invention provides a method for synthesizing oleic acid / sodium oleate vesica under an alternating electric field, belongs to the technical fields of cytobiology, membrane biophysics, biochemistry, drug carriers and micro-nano, particularly relates to a method for synthesizing the oleic acid / sodium oleate vesica, and aims to solve the problems that according to the existing vesica synthesizing methods, relatively expensive phospholipid is taken as a material, and the development of the vesica as a drug carrier is limited, or a vesica synthesized by oleic acid is smaller and is not easy to observe under an optical microscope. The method comprises the steps of 1, preparing a methanol solution of oleic acid / sodium oleate; 2, pretreating an ITO electrode; 3, coating; 4, assembling an electric forming device; 5, electrically forming the oleic acid vesica, thus finishing synthesizing the oleic acid / sodium oleate vesica under the alternating electric field. The method is used for synthesizing the oleic acid / sodium oleate vesica under the alternating electric field.

Description

technical field [0001] The invention belongs to the fields of cell biology, membrane biophysics, biochemistry, drug carrier and micro-nano technology, and specifically relates to a method for synthesizing oleic acid / sodium oleate vesicles. Background technique [0002] Vesicles have important research significance and application value in the study of primitive cell membrane models and the construction of new drug carriers because of their unique biomimetic membrane structure and the characteristics of encapsulating proteins, DNA, drugs and other chemical substances. Fatty acids and their salts are the simplest amphiphilic molecules that existed in prehistoric times. They can undergo molecular self-assembly to form vesicle structures at a specific pH value. Expensive phospholipids are used as raw materials, which limits their development as drug carriers to some extent. Oleic acid is a natural unsaturated fatty acid with low price. Its molecules exist in the state of oil-wa...

Claims

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Application Information

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IPC IPC(8): B01J13/02
Inventor 韩晓军王剑飞
Owner HARBIN INST OF TECH
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