The invention belongs to the technical field of test of optical precise imaging, and relates to a laser stimulated emission depletion (STED) and three-dimensional superresolving spectral pupil differential confocal imaging method and device. The method and device are characterized in that the spectral pupil laser differential confocal detecting technology and the laser STED imaging technology are organically integrated, the characteristics of high resolution and high scatter suppression of the spectral pupil laser differential confocal detecting technology are integrated, the laser differential confocal technology is performed to improve the axial resolution capacity, the STED technology is performed to improve the transverse resolution capacity, and as a result, the spatial resolution capacity of the system and the resistance to sample scattering can be improved. The device comprises an excitation laser system, a first dichroic mirror, a quarter-wave plate, a measuring objective lens, a sample, a scanning workbench, a quenching laser system, a beam-shaping system, a second dichroic mirror, a spectral pupil differential confocal detecting system and a data processing module. The method and device have an extensive application prospect in the micro-nano technical field with the high spatial resolution and high scattering sample suppression three-dimensional superresolving imaging and detecting capacities.