Lymph dye making silicon dioxide microparticle as supporter and its preparation method

A silica and dye technology, applied in the intersection of micro-nano technology and clinical medical foundation, can solve problems such as increasing accuracy, and achieve the effect of wide application

Inactive Publication Date: 2005-11-23
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, when de Decicco et al. performed sentinel lymph node biopsies on 250 breast cancer patients, they found that too small stains caused false positives due to excessive entry into the lymph nodes, and believed that larger particles would increase the accuracy of sentinel lymph node biopsies.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Mix 6mL cyclohexane, 1.5mL Triton X-100, 1.5mL n-hexanol and 2.5mL aqueous solution to obtain a transparent and clear microemulsion. Add an ethanol solution of ethyl orthosilicate dropwise to the microemulsion, adjust the pH to about 9 with ammonia water, and stir the reaction at room temperature. The product was separated by centrifugation, and the resulting precipitate was repeatedly and alternately washed with ethanol and deionized water, and then dispersed in deionized water.

[0027] TEM observation shows that the obtained product is mainly regular nearly monodisperse spherical silica particles with a particle size of about 70nm. However, some particles also agglomerated.

[0028]Disperse the washed precipitate in the water phase again, add isosulfur blue solution to it, disperse by ultrasonic wave for 10 minutes, let it stand still for about 30 minutes, centrifuge, discard the supernatant, and obtain a blue precipitate. By controlling the amounts of silicon diox...

Embodiment 2

[0030] Mix 6 mL of cyclohexane, 1.5 mL of Triton X-100, 1.5 mL of n-hexanol and 2.5 mL of isothiocyanate aqueous solution to obtain a transparent and clear microemulsion of isothiocyanate. Add an ethanol solution of ethyl orthosilicate dropwise to the isosulfur blue microemulsion, adjust the pH to about 9 with ammonia water, and stir overnight at room temperature. The product was separated by centrifugation, and the resulting precipitate was repeatedly and alternately washed with ethanol and deionized water, then dispersed in deionized water, and steam sterilized at 100°C for 30 min.

[0031] As a result, a blue sol embedding isosulfur blue was obtained. TEM observation showed that the obtained product was mainly regular spherical particles close to monodisperse, with a particle size of about 70nm, and a small part of the agglomerates had a particle size of submicron.

Embodiment 3

[0033] In the isosulfur blue microemulsion system of Example 2, polyethylene glycol is contained in the aqueous phase, and then silicon dioxide is synthesized by the same method as in Example 2, and the obtained silicon dioxide is dispersed in physiological saline and heated at 100° C. Steam sterilization for 30min.

[0034] As a result, a blue sol embedding isosulfur blue was also obtained. Under TEM observation, most of the particles are spherical particles with a diameter of 50-70 nm, and have good dispersion.

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PUM

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Abstract

The invention discloses a lymph dye making silicon dioxide microparticle as supporter and its preparation method, which comprises subjecting lymph gland labeling dye to adsorption, embedding to connect to the surface, porosity or internal cavity of silica dioxide particles, thus forming silica dioxide particle for labeling sentinel lymph node. The provided lymph dyestuff using silica dioxide particles as carrier agent can improve the convenience and accuracy of sentinel lymph node positioning.

Description

technical field [0001] The present invention relates to a dye with silica microparticles as a carrier and a preparation method thereof, in particular to a blue dye capable of marking lymph nodes embedded in silica microparticles or connected to the surface of silica microparticles Or the method of connecting to the pores of silica particles belongs to the intersection field of micro-nano technology and clinical medicine basis. Background technique [0002] Lymph node metastasis is the main cause of death in patients with malignant tumors. Early detection of lymph node metastasis has important clinical significance for clinical staging of tumors and corresponding treatment of tumors. [0003] Injection of reactive dyes is a common method for tracing lymph nodes during surgery. The reactive dyes used are mainly isosulfan blue, methythionium, patent blue, evans blue, indigo Card red (indigocarmine), etc., these dyes can show the general outline of lymph. Generally, lymphatic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/06
Inventor 储茂泉
Owner TONGJI UNIV
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