Phospholipid bilayer membrane array fabricated by multilayer polyelectrolyte membrane substrate and fabrication method

A polyelectrolyte and double-layer membrane technology, applied in the field of membrane biophysics, can solve the problems of expensive instruments, many control factors, and high technical level requirements, and achieve the effects of low cost, simple operation, and good controllability

Inactive Publication Date: 2013-04-10
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the process of preparing phospholipid bilayer membrane arrays, the required instruments are

Method used

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  • Phospholipid bilayer membrane array fabricated by multilayer polyelectrolyte membrane substrate and fabrication method

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Embodiment 1

[0018] (1) Preparation of APTES self-assembled monolayer film: ultrasonically clean the glass substrate with absolute ethanol and distilled water for 5 minutes, blow dry with nitrogen and place it in a plasma cleaner for 20 seconds; put the cleaned substrate into 3-ammonia Self-assemble in a mixed solution of propyltriethoxysilane (APTES) and toluene for 2 hours, and its volume ratio is 1:50. After the assembly is completed, it is cleaned with toluene and dried with nitrogen gas for use;

[0019] (2), layer-by-layer assembly of polyelectrolytes: poly(4-styrene sulfonate sodium) (PSS) and poly(diallyldimethylammonium chloride) (PDDA) were formulated to a concentration of 2 mg / mL Add NaCl to the aqueous solution to make the NaCl concentration 0.5mol / L, and adjust the pH to 1.0 for use; soak the substrate modified with 3-aminopropyltriethoxysilane (APTES) in the prepared Poly(4-styrene sulfonate sodium) (PSS) solution for 10min, take it out, rinse it with distilled water and dry ...

Embodiment 2

[0024] (1) Preparation of APTES self-assembled monolayer film: ultrasonically clean the glass substrate with absolute ethanol and distilled water for 10 minutes, blow dry with nitrogen and place it in a plasma cleaner for 30 seconds; put the cleaned substrate into 3-ammonia Self-assemble in a mixed solution of propyltriethoxysilane (APTES) and toluene for 4 hours, its volume ratio is 1:150, after the assembly is completed, it is cleaned with toluene and dried with nitrogen gas for use;

[0025] (2), layer-by-layer assembly of polyelectrolytes: poly(4-styrene sulfonate sodium) (PSS) and poly(diallyldimethylammonium chloride) (PDDA) were formulated to a concentration of 2 mg / mL Add NaCl to the aqueous solution to make the NaCl concentration 0.5mol / L, and adjust the pH to 3.0 for use; soak the substrate modified with 3-aminopropyltriethoxysilane (APTES) in the prepared In the poly(4-styrene sodium sulfonate) (PSS) solution for 20min, take it out, rinse it with distilled water and...

Embodiment 3

[0030] (1) Preparation of APTES self-assembled monolayer film: ultrasonically clean the glass substrate with absolute ethanol and distilled water for 10 minutes, blow dry with nitrogen and place it in a plasma cleaner for 30 seconds; put the cleaned substrate into 3-ammonia Self-assemble in a mixed solution of propyltriethoxysilane (APTES) and toluene for 4 hours, its volume ratio is 1:150, after the assembly is completed, it is cleaned with toluene and dried with nitrogen gas for use;

[0031] (2), layer-by-layer assembly of polyelectrolytes: poly(4-styrene sulfonate sodium) (PSS) and poly(diallyldimethylammonium chloride) (PDDA) were formulated to a concentration of 2 mg / mL Add NaCl to the aqueous solution to make the NaCl concentration 0.5mol / L, and adjust the pH to 3.0 for use; soak the substrate modified with 3-aminopropyltriethoxysilane (APTES) in the prepared In the poly(4-styrene sodium sulfonate) (PSS) solution for 20min, take it out, rinse it with distilled water and...

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Abstract

The invention relates to a phospholipid bilayer membrane array fabricated by a multilayer polyelectrolyte membrane substrate and a fabrication method. A static layer-by-layer self-assembly technology is adopted to fabricate polyelectrolyte poly-(4-sodium styrene sulfonate) (PSS) and poly-(diallyl dimethyl ammonia chloride) (PDDA) multilayer membranes on a silicon substrate modified by 3-ammonia propyltriethoxysilane, the modified substrate is subjected to patterning by a photetching technology, arrays of a polyelectrolyte in different types are fabricated, a phospholipid bilayer membrane is fabricated at the bottoms of the arrays, and the phospholipid bilayer membrane array is fabricated. According to the method, the layer-by-layer assembly technology is introduced into the fabrication of the patterned substrate, the fabrication method of the phospholipid bilayer membrane array is enriched and expanded, the method has the advantages of simplicity in operation, mild reaction conditions, good controllability, high stability, low cost and the like, and a reliable technology is provided for the research and application of biological membrane characteristics with respect to the fields of membrane biophysics, interface chemistry, biochemistry, bionics and micro-nano technologies.

Description

technical field [0001] The invention relates to membrane biophysics, in particular to a phospholipid bilayer membrane array prepared by using a multilayer polyelectrolyte membrane substrate and a method thereof. Background technique [0002] At present, methods for preparing phospholipid bilayer membrane arrays mainly include mechanical characterization, microcontact printing, photolithography, and microfluidic methods, among which photolithography has the advantages of simple operation and good controllability. However, the obstacles of the film array prepared by photolithography are mainly photoresist, aluminum oxide, etc. at present. In the process of preparing the phospholipid bilayer membrane array, the required instruments are expensive, there are many factors to be controlled, and the technical level is relatively high. Contents of the invention [0003] Based on the above shortcomings, the present invention provides a phospholipid bilayer membrane array prepared b...

Claims

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Application Information

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IPC IPC(8): C03C17/28
Inventor 韩晓军张迎齐国栋
Owner HARBIN INST OF TECH
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