A medicine-carrying chitosan slow-release microparticle algistat and a preparing method thereof

A technology of slow-release particles and chitosan, which is applied to botany equipment and methods, herbicides, algicides, biocides, etc., can solve the problems of affecting the algae inhibition effect of water bodies, easy sinking, easy expansion, etc., to achieve Not easy to expand and sink, easy to operate, good biocompatibility

Inactive Publication Date: 2019-05-21
HOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The slow-release granule algae inhibitor we prepared in the early stage has good slow-release effect, but it is easy to swell when it meets water, the particle size is large, and it is easy to sink, which affects the actual water body algae inhibition effect.

Method used

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  • A medicine-carrying chitosan slow-release microparticle algistat and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] S1, take 0.12g chitosan and dissolve in 60ml of 1% glacial acetic acid solution, and adjust its pH value to 5.1;

[0031] S2. Add 0.03 g of carboxylic acid allelopathic active substances into 40 ml of 0.75 mg / ml sodium tripolyphosphate solution, stir and mix;

[0032] S3, adding the sodium tripolyphosphate solution containing carboxylic acid allelopathic active substances dropwise to the chitosan solution at a rate of 1ml / min at normal temperature, stirring for 60min at a speed of 1000r / min on a magnetic stirrer, and Ultrasonic treatment was performed for 1 min using a sonicator;

[0033] S4. Centrifuge the prepared suspension under the condition of 12000r / min for 30min, wash the slow-release particles after centrifugation with distilled water, and repeat the centrifugal cleaning step 3 times;

[0034] S5. After adding 3% mannitol, freeze-dry to prepare drug-loaded chitosan sustained-release particles.

[0035] Acute toxicity test on aseptically cultured Microcystis a...

Embodiment 2

[0038] S1, take 0.1g chitosan and dissolve in 50ml of 1% glacial acetic acid solution, and adjust its pH value to 4.5;

[0039] S2. Add 0.02 g of carboxylic acid allelopathic active substances into 30 ml of 0.75 mg / ml sodium tripolyphosphate solution, stir and mix;

[0040] S3, adding the sodium tripolyphosphate solution containing carboxylic acid allelopathic active substances dropwise to the chitosan solution at a rate of 1ml / min at normal temperature, stirring for 60min at a speed of 1100r / min on a magnetic stirrer, and Ultrasonic treatment was performed for 1 min using a sonicator;

[0041] S4. Centrifuge the prepared suspension under the condition of 12000r / min for 30min, wash the slow-release particles after centrifugation with distilled water, and repeat the centrifugal cleaning step 3 times;

[0042] S5. After adding 4% mannitol, freeze-dry to prepare drug-loaded chitosan sustained-release particles.

[0043] Acute toxicity test on aseptically cultured Microcystis ae...

Embodiment 3

[0046] S1, take by weighing 0.2g chitosan and be dissolved in the 1% glacial acetic acid solution of 80ml, and adjust its pH value to 5.5;

[0047] S2. Add 0.04 g of carboxylic acid allelopathic active substances into 50 ml of 0.75 mg / ml sodium tripolyphosphate solution, stir and mix;

[0048] S3, adding the sodium tripolyphosphate solution containing carboxylic acid allelopathic active substances dropwise into the chitosan solution at a rate of 1ml / min at room temperature, stirring for 60min at a speed of 1200r / min on a magnetic stirrer, and Ultrasonic treatment was performed for 1 min using a sonicator;

[0049] S4. Centrifuge the prepared suspension under the condition of 12000r / min for 30min, wash the slow-release particles after centrifugation with distilled water, and repeat the centrifugal cleaning step 3 times;

[0050] S5. After adding 3% mannitol, freeze-dry to prepare drug-loaded chitosan sustained-release particles.

[0051] Acute toxicity test on aseptically cul...

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Abstract

A medicine-carrying chitosan slow-release microparticle algistat and a preparing method thereof are disclosed. The algistat includes slow-release microparticles and a carboxylic acid type active allelochemical embedded in the microparticles. The slow-release microparticles are formed from chitosan amino cations and sodium tripolyphosphate polyanion through ion crosslinking reaction. Through combination of micro-nano techniques, chitosan and sodium tripolyphosphate are adopted as carrier materials to embed the carboxylic acid type algae suppression active substance to prepare the slow-release microparticle algistat which has a small particle size and which is not liable to expand or sink. The Microcystis aeruginosa suppressing time of the carboxylic acid type active allelochemical in waterbodies is prolonged, the cost is reduced, and a problem that direct addition of the carboxylic acid type active allelochemical into a water body has defects of large active substance dosage, easy loss, incapability of reaching an algae suppression concentration, and the like can be effectively solved. The adopted chitosan and the sodium tripolyphosphate are natural nontoxic biodegradable polymer materials good in biocompatibility, and can effectively avoid secondary pollution in algae suppression processes. The algistat can be widely applied for natural water bodies such as ponds, reservoirs and lakes.

Description

technical field [0001] The invention relates to an algae inhibitor, in particular to a drug-loaded chitosan slow-release particulate algae inhibitor and a preparation method thereof, belonging to the technical field of water body eutrophication inhibition of algae outbreak. Background technique [0002] Microcapsules are tiny particles made of natural or synthetic polymer membranes that wrap solid, liquid or gaseous functional materials. Under proper conditions, the wall material will be destroyed and the core material will be released. Since the 1950s, microencapsulation technology has attracted people's attention. By the 1980s, microcapsule technology was further developed, and nano-microcapsule technology appeared, making the research on microcapsule technology one of the focuses of people's attention. Microcapsule technology has become a research hotspot in the fields of materials, chemistry, food, chemical engineering, medicine, and biotechnology because of its ability...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N25/28A01P13/00A01N37/36A01N37/40A01N37/04
Inventor 倪利晓顾国秀胡玲玲荣诗怡刘烜瑜王娜岳菲菲
Owner HOHAI UNIV
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