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A method for preparing pharmaceutical grade l-tryptophan

A tryptophan and pharmaceutical-grade technology, applied in the field of preparation of pharmaceutical-grade amino acids, can solve the problems of large solvent consumption, low crystallization concentration, and inability to realize large-scale industrial production, so as to reduce the amount of solvent used and improve light transmission efficiency and the effect of less solvent usage

Active Publication Date: 2017-11-17
BENGBU BBCA MEDICINE SCI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, it solves the shortcomings of too much solvent consumption and low crystallization concentration in the refining process of L-tryptophan, which makes it impossible to realize large-scale industrial production.

Method used

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  • A method for preparing pharmaceutical grade l-tryptophan
  • A method for preparing pharmaceutical grade l-tryptophan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053]1) Dissolution: Take 50kg of crude feed-grade L-tryptophan and put it into a 200L enamel kettle, add 100kg of 5% sodium hydroxide aqueous solution (the weight ratio of solvent: crude product is 10:5), stir evenly, and heat to 60°C Stir with heat preservation to dissolve all the L-tryptophan.

[0054] 2) Decolorization: Add 2.5kg of activated carbon (equivalent to 5% of the weight of feed-grade L-tryptophan crude product) after dissolving, keep warm at 60°C for 0.5h, filter through a 0.22μm titanium rod while it is hot, and pour it into a 200L crystalline enamel kettle , a colorless and clear filtrate was obtained.

[0055] 3) pH adjustment: cooling the decolorized filtrate to 50° C., and then adjusting the pH of the filtrate to 6.0 with 10% hydrochloric acid to produce a large amount of white crystals of L-tryptophan.

[0056] 4) Filtration and washing with water: cool the crystals obtained in step 3) to room temperature while stirring, and centrifuge for 20 minutes (ce...

Embodiment 2

[0059] 1) Dissolution: Take 60kg of crude feed-grade L-tryptophan and put it into a 200L enamel kettle, add 100kg of 5% sodium hydroxide aqueous solution (the weight ratio of solvent: crude product is 10:6), stir evenly, and heat to 50°C Stir with heat preservation to dissolve all the L-tryptophan.

[0060] 2) Decolorization: Add 3kg of activated carbon (equivalent to 5% of feed grade L-tryptophan crude product) after dissolving, keep it warm at 50°C for 0.5h, filter it through a 0.22μm titanium rod while it is hot, and pour it into a 200L crystalline enamel kettle. A colorless and clear filtrate was obtained.

[0061] 3) pH adjustment: cooling the decolorized filtrate to 45° C., and then adjusting the pH of the filtrate to 6.4 with 10% hydrochloric acid to produce a large amount of white crystals of L-tryptophan.

[0062] 4) Filtration and washing with water: cool the crystals obtained in step 3) to room temperature while stirring, and centrifuge for 20 minutes (centrifuge s...

Embodiment 3

[0065] 1) Dissolution: Take 60kg of crude feed-grade L-tryptophan and put it into a 200L-enamel kettle, add 100kg of 8% sodium hydroxide aqueous solution (the weight ratio of solvent: crude product is 10:6), stir evenly, and heat to 55 ℃ and keep stirring to dissolve all the L-tryptophan.

[0066] 2) Decolorization: add 3kg of activated carbon (equivalent to 5% of the weight of feed-grade crude L-tryptophan) after dissolving, keep warm at 55°C for 0.5h, and filter it through a 0.22μm titanium rod while it is hot into a 200L crystalline enamel kettle , a colorless clear filtrate was obtained.

[0067] 3) Adjusting the pH: cooling the decolorized filtrate to 40° C., then adjusting the pH of the filtrate to 5.4 with 10% hydrochloric acid to produce a large amount of white crystals of L-tryptophan.

[0068] 4) Filtration and washing with water: cool the crystals obtained in step 3) to room temperature while stirring, and centrifuge for 20 minutes (centrifuge speed 1700rpm) to obt...

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Abstract

The invention relates to a process for preparing pharmaceutical-grade L-tryptophan, which comprises the steps of dissolving, decolorizing, adjusting pH, filtering, washing, and drying. Wherein the dissolving is to dissolve the crude product of L-tryptophan in the solvent, in terms of weight ratio, the solvent: crude product is 100:50-60. The process of the invention is simple, and the refined L-tryptophan complies with the standard of the Chinese Pharmacopoeia of the 2010 edition. The process of the invention can realize large-scale refined production of L-tryptophan, which solves the shortcomings of excessive solvent consumption, low crystallization concentration and difficulty in large-scale industrial production in the L-tryptophan refining process.

Description

technical field [0001] The invention relates to a preparation method for preparing pharmaceutical-grade amino acids, in particular to a method for refining L-tryptophan to prepare pharmaceutical-grade L-tryptophan. Background technique [0002] The chemical name of tryptophan is β-indolylalanine, which was isolated from casein in 1902. Tryptophan has 3 isomers, which are L-type, D-type and racemic DL-type. Tryptophan is an important nutritionally essential amino acid that can only be synthesized by microorganisms or plants. Among them, L-tryptophan is one of the 8 kinds of amino acids necessary for the human body. Humans and animals cannot synthesize it by themselves and can only be ingested from food. D-tryptophan hardly metabolizes in the human body and is non-toxic, so the human body does not need to supplement it. As an essential amino acid, L-tryptophan is widely used in medicine, food and feed. The structural formula of L-tryptophan is: [0003] [0004] L-tryp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 韦亚锋林文龙陈昀张瑾胡媛
Owner BENGBU BBCA MEDICINE SCI DEV