Extraction method of Yunzhi polysaccharide
A technology of Yunzhi polysaccharide and extraction method, which is applied in the directions of drug combination and anti-tumor drug, can solve the problems of high impurity content and backward extraction process, and achieve the effect of strong anti-tumor activity, ensuring the purity of the finished product, and ensuring the color and luster.
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Embodiment 1
[0015] 1) Crush Yunzhi, extract 2 times with 6 times the weight of 80v% ethanol, reflux for 2h each time, filter, and take the filter residue for later use;
[0016] 2) the filter residue is extracted 2 times with 6 times the weight of water, each time is extracted for 2h, filtered, and the filtrate is collected for subsequent use;
[0017] 3) Concentrating the filtrate to an extract with a relative density of 1.1 at 60° C., adding 90v% ethanol to the extract until the ethanol content is 80v%, and centrifuging to obtain a precipitate;
[0018] 4) Wash the precipitate with 2 times the weight of absolute ethanol and acetone respectively, dissolve the washed precipitate with 2 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 1 million, collect the concentrated solution, and then pass the concentrated solution through the interception. Hollow fiber membrane with a molecular weight of 1.5 million, collect the permeate, centrifuge, fi...
Embodiment 2
[0026] 1) Crush Yunzhi, extract 3 times with 12 times the weight of 95v% ethanol, reflux for 3h each time, filter, and take the filter residue for later use;
[0027] 2) the filter residue is extracted 3 times with 12 times the weight of water, and each time is extracted for 3h, filtered, and the filtrate is collected for subsequent use;
[0028] 3) Concentrating the filtrate to an extract with a relative density of 1.3 at 60° C., adding 95v% ethanol to the extract until the ethanol content is 85v%, and centrifuging to obtain a precipitate;
[0029] 4) Wash the precipitate with 4 times the weight of absolute ethanol and acetone in turn, dissolve the washed precipitate with 4 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 1 million, collect the concentrated solution, and then pass the concentrated solution through the interception. Hollow fiber membrane with a molecular weight of 1.5 million, collect the permeate, centrifuge, f...
Embodiment 3
[0032] 1) Crush Yunzhi, extract 2 times with 10 times the weight of 90v% ethanol, reflux for 2h each time, filter, and take the filter residue for later use;
[0033] 2) the filter residue is extracted 2 times with 10 times the weight of water, each time is extracted for 2h, filtered, and the filtrate is collected for subsequent use;
[0034] 3) Concentrating the filtrate to an extract with a relative density of 1.2 at 60° C., adding 95v% ethanol to the extract until the ethanol content is 85v%, and centrifuging to obtain a precipitate;
[0035] 4) Wash the precipitate with 3 times the weight of absolute ethanol and acetone successively, dissolve the washed precipitate with 3 times of distilled water, pass through a hollow fiber membrane with a molecular weight cut-off of 1 million, collect the concentrated solution, and then pass the concentrated solution through the interception. Hollow fiber membrane with a molecular weight of 1.5 million, collect the permeate, centrifuge, ...
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