Tobacco black shank resistance single plant identification method

Abstract

The invention discloses a tobacco black shank resistance single plant identification method. The method includes the steps that tobacco which is about to be inoculated and grows orderly is obtained through tissue culturing rapid propagation, application inoculation processing is conducted, culture medium slab patches overgrown with black shank hyphae are attached to the rootstocks of microtrauma tobacco, the treated tobacco is cultivated in a humidity-controllable phytotron, resistance distinguish is conducted according to a research result generated 14 days after inoculation is conducted, and the resistance is divided according to the morbidity a. The immune or highly-resistant morbidity a is equal to zero, the disease-resistant morbidity a is larger than zero and smaller than or equal to 25%, the moderately-resistant morbidity a is larger than 25% and smaller than or equal to 45%, the moderately-susceptible morbidity a is larger than 45% and smaller than or equal to 65%, the susceptible morbidity a is larger than 65% and smaller than or equal to 80%, the highly-susceptible morbidity a is larger than 80% and smaller than or equal to 100%, and the immune or highly-resistant, disease-resistant and moderately-resistant materials are resistance materials. The method is high in identification result reproducibility, good in stability, high in efficiency, capable of achieving large-scale resistance identification and especially suitable for screening disease-resistant target single plants from a huge number of mutagenesis populations or mutant population, hybridization variation populations.

Description

technical field

[0001] The invention belongs to the technical field of tobacco disease resistance detection, and in particular relates to an identification method for tobacco black shank resistant individual plants. Background technique

[0002] Tobacco black shank is caused by the fungus Phytophthoranicotianae, and once infested, the entire tobacco plant dies. Under natural conditions, its pathogen Phytophthora nicotiana mainly infects underground parts such as roots and stems, and occasionally leaves are infected. Root or root shin inoculation is often used in variety resistance identification, and indoor inoculation identification (GB / T23224), which is widely used, requires 10 to 20 plants per material to effectively evaluate resistance. This method is obviously not suitable for the identification of individual plant resistance of a large number of mutagenic populations, mutant populations, and hybrid mutant populations. Therefore, it is necessary to establish a new met...

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