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Polypeptide for inhibiting mTOR and application thereof

A technology for inhibiting polypeptides and controlled release preparations, applied in the field of medicine

Inactive Publication Date: 2015-11-25
SUZHOU PULUODA BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no well-developed mTOR inhibitory polypeptides available for the prevention or treatment of diabetes

Method used

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  • Polypeptide for inhibiting mTOR and application thereof
  • Polypeptide for inhibiting mTOR and application thereof
  • Polypeptide for inhibiting mTOR and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] In Vitro Cell Viability Assay of mTOR Inhibiting Polypeptides

[0013] The MTT colorimetric method was used. Insulinoma cells (INS-1 cells) with logarithmic growth were added to 96-well culture plate at 1.0×105, and cultured for 24 hours. Experimental wells and positive drug control wells were respectively added with different concentrations of experimental drug mTOR inhibitory polypeptide; blank group was added same volume of solvent. Set up five duplicate wells in every well, culture 48h, add MTT in every hole, after 4h of action, add DMSO, hatch 30min, measure the absorbance A value at 570nm place of microplate reader, according to the formula cell growth proliferation rate=(experimental group absorbance value / Absorbance value of control group -1)×100%. The calculated EC50 of the experimental drug was 20.76nmol / L.

Embodiment 2

[0015] In vivo hypoglycemic experiment of mTOR inhibitory polypeptide

[0016] Kunming mice were fed with high-fat and high-sugar to a body weight of 18-22 g, half male and half female, fasted for 24 hours before the experiment, and intraperitoneally injected with streptozotocin (STZ) 50 mg / kg (1% citrate buffer). liquid solution preparation). One week later, blood glucose (BS) was measured by blood collection from the tail vein of the mice. If the BS was higher than 16mmol / L, the modeling was successful. After modeling, the mice were divided into 3 groups, 10 in each group. Diabetes model group (DM group): subcutaneous injection of the same amount of PBS buffer; control group (Bai Sugar): 20 μg / kg subcutaneous injection dissolved in 0.1mol / LPBS buffer), twice a day for 15 consecutive days; sample group (mTOR inhibitory polypeptide): the same as the control group; another 10 normal mice were taken as the blank group. On the 15th day after administration, blood was taken from...

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Abstract

The invention relates to the field of medicines, in particular relates to polypeptide which can inhibit mTOR and prevent and treat diabetes. The sequence of the polypeptide is EIQATWYEKLHEW. The application of the polypeptide in preventing or treating the diabetes can be realized by multiple administration methods including subcutaneous injection or intramuscular injection, intravenous injection or intravenous drip, oral administration such as pills or capsules, nasal sprays and the like. The polypeptide for inhibiting mTOR and application thereof have the beneficial effects that the mTOR can be inhibited in a targeted manner, the physiological and pathological effects generated by an mTOR receptor can be inhibited, and the effects of preventing and treating the diabetes can be achieved.

Description

Technical field: [0001] The invention relates to the field of medicine, in particular to a polypeptide for treating diabetes. Background technique [0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia. It is a syndrome of glucose, protein and lipid metabolism disorders caused by insufficient insulin or defects in insulin cell metabolism. In recent years, the number of diabetic patients has gradually increased, and is divided into type I diabetes and type II diabetes. Among them, the number of patients with type II diabetes accounts for 85-90% of the total number of diabetes, which seriously threatens the health and life of patients. If diabetes is not well controlled, it may cause many complications, such as diabetic nephropathy, retinopathy, high blood pressure, etc. [0003] At present, most patients treat diabetes by injecting insulin or oral hypoglycemic drugs to help control blood sugar in the body, or regulating insulin secretion....

Claims

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Application Information

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IPC IPC(8): C07K7/08A61K38/10A61P3/10
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
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