Method for preparing (R)-3-phenyl-indanol through tarlaromyces flavus
A technology of yellow-blue-like bacteria and phenylindanone is applied in the field of yellow blue-like bacteria cell biocatalytic preparation of chiral pharmaceutical intermediate-3-phenyl-indanol, which can solve the problem of addition, low conversion rate, and difficulty in industrialized production. And other issues
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Embodiment 1
[0020] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.
[0021] The ingredients of the culture solution are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-4g / L, yeast extract 8-10g / L, glucose 18-22g / L, malt extract 20- 25g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.
[0022] The preparation process of the wet cyanobacteria cells is as follows: the cyanobacterium chrysogenum ATCC18325 was cultured on an inclined plane and shake flasks to obtain a seed liquid. Add culture medium to 1L fermenter; add culture medium to fermenter, autoclave at 121°C for 30 minutes, coo...
Embodiment 2
[0025] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.
[0026] The ingredients of the culture solution are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-4g / L, yeast extract 8-10g / L, glucose 18-22g / L, malt extract 20- 25g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.
[0027] The preparation process of the wet cyanobacteria cells is as follows: the cyanobacterium chrysogenum ATCC18325 was cultured on an inclined plane and shake flasks to obtain a seed solution. Add culture medium to a 5L fermenter; add culture medium to a fermenter, autoclave at 121°C for 30 minute...
Embodiment 3
[0030] The ingredients of the seed medium are: corn steep liquor (calculated as dry matter) content of 35-45g / L, yeast extract 10g / L, glucose 20g / L, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L L, potassium dioxyphosphate 1.8-2.3g / L; add 15g / L agar powder when preparing solid medium.
[0031]The ingredients of the culture solution are: corn steep liquor (calculated as dry matter) content of 35-45g / L, soybean powder 3-4g / L, yeast extract 8-10g / L, glucose 18-22g / L, malt extract 20- 25g, ammonium sulfate 0.5-0.7g / L, magnesium sulfate 0.3-0.4g / L, potassium dioxyphosphate 1.8-2.3g / L, calcium carbonate 0.7g / L, ferrous sulfate 0.18g / L, manganese sulfate 0.025g / L;pH5.3.
[0032] The preparation process of the wet cyanobacteria cells is as follows: the cyanobacterium chrysogenum ATCC18325 is cultivated on an inclined plane and shaken to obtain a seed solution. Add culture medium to 10L fermenter; add culture medium to fermenter, autoclave at 121°C for 30 minutes, cool t...
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