Fast screening method of pig feed non-starch polysaccharide enzyme

A non-starch polysaccharide enzyme and non-starch polysaccharide technology, which is applied in the field of rapid screening of non-starch polysaccharide zymograms in pig feed, can solve the problems of many factors affecting systematic errors and accidental errors, cost, and lack, and achieve rapid optimization of non-starch polysaccharides Zymogram, overcoming blindness, effect of improving efficacy

Active Publication Date: 2015-11-25
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The in vivo method is to conduct animal experiments, which is often costly, laborious, and time-consuming, and there are many factors affecting systematic errors and accidental errors.
The in vitro method has the advantages of rapidity, simplicity, and low cost, but the parameters and conditions selected by the in vitro method often lack theoretical explanations and are somewhat arbitrary (Coles et al., 2005)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fast screening method of pig feed non-starch polysaccharide enzyme
  • Fast screening method of pig feed non-starch polysaccharide enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Such as figure 1 As shown, the specific implementation method of the present invention can be implemented in the following manner:

[0022] Select corn-soybean meal-type compound feed and put it into a pulverizer, wherein the feed includes corn-soybean meal-type compound feed and corn and soybean meal raw materials that make up the compound feed; then control the speed of the pulverizer at 8000-10000 rpm per minute, and the crushing time is 5 to 10 minutes; pass the crushed feed through a 0.25 to 0.42 mm sieve; accurately weigh 100 to 250 mg of feed sample, and measure the content of non-starch polysaccharide components in compound feed and feed raw materials;

[0023] According to the non-starch polysaccharide components of the compound feed and feed raw materials, according to the results of principal component analysis, weigh 1 to 2 grams of corn-soybean meal compound feed, and add three kinds of non-starch polysaccharide enzymes, i.e. wood Glycanase, cellulase, β-g...

Embodiment 2

[0030] Select the wheat-soybean meal type compound feed and put it into a micro pulverizer, wherein the feed includes wheat-soybean meal type compound feed and the wheat and soybean meal raw materials that make up the compound feed; then control the speed of the micro pulverizer at 8000~ 10,000 rpm, crushing time is 5-10 minutes; pass the crushed feed through a 0.25-0.42 mm sieve; accurately weigh 100-250 mg of feed sample, and measure the content of non-starch polysaccharide components in compound feed and feed raw materials;

[0031] According to the non-starch polysaccharide components of the compound feed and feed raw materials, according to the results of principal component analysis, weigh 1 to 2 grams of wheat-soybean meal compound feed, and add four kinds of non-starch polysaccharide enzymes in the feed, namely wood Glycanase, cellulase, β-glucanase, pectinase, the concentration gradient levels of each non-starch polysaccharide enzyme additive were set to 0, 100, 200, 3...

Embodiment 3

[0038] (1) Choose a corn-soybean meal-cotton meal-rape meal ration, and add six kinds of non-starch polysaccharases, namely xylanase, cellulase, β-glucanase, β-mannose, respectively, to the feed Glycanase, arabinanase and pectinase, the concentration gradient levels of each non-starch polysaccharide enzyme additive were set to 0, 100, 200, 300, 400, 500, 600, 700, 800, 900 μg / g, Prepared a series of corn-soybean meal-cotton meal-rape meal type diets added with different concentrations of non-starch polysaccharidase;

[0039] Select corn-soybean meal-cotton meal-rape meal type compound feed and put it into the pulverizer, wherein said feed comprises corn-soybean meal-cotton meal-rape meal type compound feed and the corn, soybean meal, cotton meal, rapeseed meal that make up the compound feed Meal raw material; then control the speed of the micro pulverizer at 8,000 to 10,000 rpm, and the pulverization time is 5 to 10 minutes; pass the pulverized feed through a 0.25 to 0.42 mm s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A fast screening method of pig feed non-starch polysaccharide enzyme includes: analyzing feed non-starch polysaccharide components, setting non-starch polysaccharide enzyme type and dosage, simulating feed gastrointestinal tract digestion, and optimizing the non-starch polysaccharide enzyme. The method has the advantages that the non-starch polysaccharide enzyme component analyzing technology and the in vitro digestion simulation method are used, feed in vitro energy is used to represent the effect of the non-starch polysaccharide enzyme, and the optimal non-starch polysaccharide enzyme added into feed is obtained fast.

Description

technical field [0001] The invention relates to a rapid screening method for the non-starch polysaccharide zymogram of pig feed, in particular to a method for rapidly optimizing the non-starch polysaccharide zymogram of feed by analyzing the non-starch polysaccharide component of feed and using an in vitro simulated digestion method. Background technique [0002] The main component of livestock and poultry feed is carbohydrates, which provide the main energy for animals. As feed costs continue to rise, cheaper feed ingredients (such as by-products of grain processing, corn DDGS, sorghum DDGS) are constantly being sought to partially replace traditional corn and soybean meal feed ingredients. However, these feed ingredients often contain relatively high non-starch polysaccharides, some of which are difficult to be fully utilized by the animal body (Anguita et al., 2006); although the other part can be fermented by the large intestine of livestock and poultry to produce volati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/44C12Q1/40C12Q1/37C12Q1/34
Inventor 陈亮张宏福高理想
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap