A method of using hormone content to control the high proliferation rate of Phalaenopsis

Abstract

The invention relates to a method for controlling the qualified rate of high proliferation of Phalaenopsis by using hormone content, which comprises operation steps, sampling and disinfection, induction of buds, proliferation of buds, planting culture, and rooting culture, including two steps in the proliferation stage of buds , a. Inoculate the buds that have grown up and can be separated from the stem segment into the medium of 1 / 2MS + cytokinin 0.3 ~ 0.35mg + naphthaleneacetic acid 0.03 ~ 0.035mg + potatoes 100g / l + white sugar 15g / l + agar 4.7g / l B. When the buds proliferate and produce side buds, they are inoculated in the culture medium of 1 / 2MS+cytokinin 0.2~0.25mg+naphthaleneacetic acid 0.02~0.025mg+potatoes 100g / l+white sugar 15g / l+agar 4.7g / l to carry out proliferation and subculture Cultivation, the method of the invention ensures the high proliferation rate of Phalaenopsis clustered buds and low defective seedling rate, thereby producing high-quality seedlings faster.

Description

technical field [0001] The invention relates to the breeding of Phalaenopsis, in particular to a method for controlling the qualified rate of high proliferation of Phalaenopsis by using hormone content. Background technique [0002] Phalaenopsis, also known as Phalaenopsis, is one of the most widely cultivated and popular species in Orchidaceae. Due to its large flowers and long flowering period, some varieties can bloom for several months. Unique and beautiful, deeply loved by people all over the world. With the progress of society and the development of economy, people's material and cultural life is constantly enriched. The flower industry, especially a batch of so-called "ocean orchids" such as Phalaenopsis, are gradually accepted and loved by consumers. However, there is a serious problem in the Phalaenopsis industry. Problems: few wild resources, lack of fine varieties of resources. [0003] Hybrid breeding is still the most commonly used method in Phalaenopsis breed...

AI Technical Summary

Problems solved by technology

With the progress of society and the development of economy, people's material and cultural life is constantly enriched. The flower industry, especially a batch of so-called "ocean orchids" such as Phalaenopsis, are gradually accepted and loved by consumers. However, there is a serious problem in the Phalaenopsis industry. Problems: Few wild resources, lack of fine varieties

[0005] At present, the proliferation rate of Phalaenopsis tissue culture materials is about 2~2.3, but according to statistics, the proportion of vitrified buds is nearly 10%, and nearly 8% of tissue culture materials have difficulties in differentiation, and a total loss of tissue culture materials of nearly 20%