Collagen composition for improving allergy symptoms and collagen patch

A technology of collagen and composition, which is applied in the field of biomedicine and can solve problems such as human harm

Active Publication Date: 2015-12-02
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of allergic symptoms mainly relies on internal medicine or externally applied hormone dr

Method used

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  • Collagen composition for improving allergy symptoms and collagen patch
  • Collagen composition for improving allergy symptoms and collagen patch
  • Collagen composition for improving allergy symptoms and collagen patch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Preparation of mesenchymal stem cell extract

[0063] The primary human umbilical cord or umbilical cord blood-derived mesenchymal stem cells were isolated and cultured and then subcultured. The culture medium used for primary culture and subculture was DMEM / F12 medium + 10% fetal bovine serum. When the cell confluence reaches 80%, discard the culture medium, then wash the cells with PBS phosphate buffer three times, then add 0.04% EDTA + 0.25% trypsin digestion solution, the amount of digestion solution is 2mL / 10cm 2 The bottom area of ​​the petri dish, after 80% of the cells become round, add the culture solution to terminate the digestion, and the added volume of the culture solution is 5 times the added volume of the digestion solution. Centrifuge the digested liquid at 600g for 5 minutes, remove the supernatant, add the culture medium to resuspend the cells, and adjust the cell density to 1×10 5 / mL, the cell suspension was added to a new dish, recorded ...

Embodiment 2

[0064] Example 2 Preparation of collagen composition

[0065] Take the P3~P8 generation cells, discard the culture medium and wash 3 times with PBS, according to 2mL / 10cm 2 0.04% EDTA+0.25% trypsin digestion solution was added to the ratio of the bottom area of ​​the petri dish to digest the cells, and then the digested cell suspension was sonicated at 9,000 cycles with a sonicator for 8 minutes.

[0066] After the treatment, the suspension was centrifuged at 19000 g for 25 minutes, the supernatant was discarded, PBS containing 500 mmol NaCl was added to resuspend the precipitate, and the resuspended liquid was filtered through a 0.45 μm pore size filter.

[0067] The filtrate was collected and concentrated by ultrafiltration using a filter membrane with a molecular weight cut-off of 5KD, and the concentrated volume was 1 / 5 of the original volume.

[0068] The concentrated solution was filtered and sterilized with a 0.22um μm pore size filter, and then the protein concentrati...

Embodiment 3

[0071] Example 3 Preparation of collagen composition

[0072] Take the P3~P8 generation cells, discard the culture medium and wash 3 times with PBS, according to 2mL / 10cm 2 0.04% EDTA+0.25% trypsin digestion solution was added to the ratio of the bottom area of ​​the petri dish to digest the cells, and then the digested cell suspension was sonicated at 9,000 cycles with a sonicator for 8 minutes.

[0073] After the treatment, the suspension was centrifuged at 15000 g for 25 minutes, the supernatant was discarded, PBS containing 700 mmol NaCl was added to resuspend the precipitate, and the resuspended liquid was filtered through a 0.5 μm pore size filter.

[0074] The filtrate was collected and concentrated by ultrafiltration using a filter membrane with a molecular weight cut-off of 5KD, and the concentrated volume was 1 / 10 of the original volume.

[0075]The concentrated solution was sterilized by filtration with a 0.22um μm pore size filter, and then the protein concentrati...

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Abstract

The invention provides a collagen composition for improving allergy symptoms. The collagen composition comprises collagen, a mesenchymal stem cell extract, ascorbate, aspartate, sodium citric, sorbitol and trehalose according to a mass ratio of (5-10): (0.0005-0.002): (1.2-2.2): (1.8-2.8): (0.1-0.5): (0.4-1.4): (0.05-0.3). By the collagen composition, skin allergy symptoms can be reduced, and skin conditions can be improved. Moreover, the collagen composition does not have toxic or side effects on human bodies, and is high in safety. The invention also provides a collagen patch.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a collagen composition and a collagen film patch for improving allergic symptoms. Background technique [0002] In recent years, reported cases of skin allergies caused by exposure to allergens have shown an increasing trend year by year, and the top three allergens are pollen, dust and cosmetics. Especially cosmetics, serious can cause skin damage and even leave permanent scars. The collagen in the collagen film that eliminates acne, promotes wound healing, and reduces scarring is also an allergen. In clinical cases, patients have reported severe allergic symptoms caused by collagen injection. [0003] At present, the treatment of allergic symptoms mainly relies on internal medicine or externally applied hormone drugs, but both internally administered drugs and externally applied hormone drugs will cause certain harm to the human body. Contents of the inventio...

Claims

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Application Information

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IPC IPC(8): A61K38/39A61P37/08A61P17/00A61K35/28
Inventor 陈海佳王一飞葛啸虎万桦王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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