Probiotic composition for improving allergic diseases, probiotic composition bacterial powder, preparation method and application thereof

A technology of allergic diseases and probiotics, applied in the direction of allergic diseases, biochemical equipment and methods, and methods based on microorganisms, to achieve the effects of improving allergic symptoms, promoting functional effects, intestinal cleaning and protection

Inactive Publication Date: 2020-08-18
郑州和合生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus johnsonii has a long history of safe use, is considered non-pathogenic, and has various probiotic benefits, but it has not been used for the improvement of allergic diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of strains

[0025] Isolated from the intestinal tract of healthy mice, cultivated on nutrient agar solid medium at 36-38°C for 24-48 hours, and separated by streaking multiple times to obtain a single colony. , generally ruled, facultatively anaerobic, sometimes microaerobic, poor growth in aerobic conditions, better growth in reduced oxygen tension.

[0026] 2. Identification of strains

[0027] Isolate and purify the strains, use the 16SrRNA gene as a Marker fragment, amplify the 16SrRNA sequence in the gene with universal primers, and then perform electrophoresis detection, use a 3730XL sequencer to perform a generation of paired-end sequencing to obtain abi sequencing peak map files, and assemble them through software. Comparing with the NT database to obtain the information of close relatives, it is Lactobacillus johnsonii, and the strain number is LBJ 456 TM , its 16SrRNA characteristic sequence is shown in SEQ ID NO.1:

[0028]GAGTTTGGATCCTGGCT...

Embodiment 2

[0043] The difference between this embodiment and embodiment 1 is:

[0044] 4. Preparation of probiotic composition bacterial powder for improving allergic diseases

[0045] Wherein: the probiotic composition for improving allergic diseases includes the following components by weight percentage: 95% probiotic A and 5% probiotic B;

[0046] Wherein: probiotic A is Lactobacillus johnsonii, and the Lactobacillus johnsonii is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20191013. The probiotic B includes Lactobacillus reuteri; wherein: Lactobacillus reuteri specifically selects Lactobacillus reuteri with the number PB-LR09.

[0047] The probiotic composition bacterium powder for improving allergic diseases includes the bacterium powder and auxiliary materials prepared from the probiotic composition; wherein: the auxiliary materials include fructooligosaccharides.

[0048] The preparation method of the probiotic composition bact...

Embodiment 3

[0056] 4. Preparation of probiotic composition bacterial powder for improving allergic diseases

[0057] Wherein: the probiotic composition for improving allergic diseases includes the following components by weight percentage: 50% probiotic A and 50% probiotic B;

[0058]Wherein: probiotic A is Lactobacillus johnsonii, and the Lactobacillus johnsonii is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20191013. The probiotics B include Lactobacillus casei, Lactobacillus paracasei and Lactobacillus reuteri; wherein: the specific selection number of Lactobacillus casei is Lactobacillus casei PB-LC39, and the specific selection number of Lactobacillus paracasei is HH-LP58 Lactobacillus paracasei and Lactobacillus reuteri specifically select the Lactobacillus reuteri whose number is PB-LR09.

[0059] The probiotic composition bacteria powder for improving allergic diseases includes the bacteria powder and auxiliary materials prepar...

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PUM

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Abstract

The invention discloses a probiotic composition for improving allergic diseases, probiotic composition bacterial powder, a preparation method and an application thereof, and belongs to the technical field of probiotic healthy food. The probiotic composition comprises the following components in percentage by weight: 50%-95% of probiotics A and 5%-50% of probiotics B; wherein the probiotics A are lactobacillus johnsonii, the lactobacillus johnsonii is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20191013; lactobacillus johnsonii can stimulatereceptors on dendritic cells on the intestinal wall and bind the receptors, the translated proteins in the cells are activated and activated to move into the nucleus to release a large amount of cytokines, by promoting secretion increase of interleukin 12 (IL-12) and interferon gamma, allergic symptoms are effectively improved, and immune globulin IgE is inhibited by stimulating intestinal tractsto regulate Th1-type immune response, thereby improving the allergic phenomenon of excessive Th1-type and Th2-type immune response.

Description

technical field [0001] The invention belongs to the technical field of probiotic health food, and in particular relates to a probiotic composition for improving allergic diseases, a probiotic composition bacterial powder, a preparation method, and an application. Background technique [0002] For example, allergic diseases, perennial allergic rhinitis and asthma are the most common allergic diseases; probiotics are live microorganisms that can prevent and improve allergic diseases, mainly by stimulating the growth of dendritic cells on the intestinal wall. The receptor binds, activates and activates the translation protein in the cell to move to the nucleus to release a large amount of cytokines, which can effectively improve the allergic symptoms by promoting the secretion of interleukin 12 (IL-12) and interferon γ, and stimulate intestinal It regulates Th1 type immune response and inhibits immunoglobulin IgE, thereby improving the hypersensitivity phenomenon of Th1 and Th2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P37/08C12R1/225C12R1/245C12R1/25
CPCA61K35/747A61P37/08C12N1/20C12N1/205C12R2001/225A61K2300/00
Inventor 丁一罗伯特·席斯特尔宋士良闫屹泓
Owner 郑州和合生物工程技术有限公司
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