A preparation method of nanoparticles with strong catalase activity

Abstract

The invention discloses a preparing method for nanometer particles with high hydrogen peroxide enzyme activity. The method comprises the following steps that 1, a chloroauric acid solution is boiled in a continuously-stirring mode and flows back, and meanwhile, a sodium citrate solution is added at a constant speed so as to reduce chloroauric acid to form gold nanometer particles with the grain size of 12-14 nm; 2, a mixed solution of chloroauric acid and chloroplatinic acid is added into the material prepared in the step 1, then an ascorbic acid solution is slowly added, and the mixture is subjected to a reaction for 2-12 hours at the stirring speed of 100-800 rpm and at the temperature of 10 DEG C-50 DEG C, so that the gold nanometer particles are wrapped with a gold platinum double-metal shell layer to obtain the nanometer particles with the high hydrogen peroxide enzyme activity. Compared with a traditional method, the method is simple, efficient and high in universality, and the synthesized nanometer particles have high and stable hydrogen peroxide enzyme activity.

Description

technical field [0001] The invention belongs to the technical field of bioactive nanometer materials, and in particular relates to a preparation method of nanoparticles with strong catalase activity. Background technique [0002] Catalase is a protease ubiquitous in organisms that can catalyze the decomposition of hydrogen peroxide to produce oxygen and water. (George, P.Reaction between Catalase and Hydrogen Peroxide[J].Nature 1947,160,41-43; Lemberg,R.; Foulkes,E.C.Reaction between Catalase and Hydrogen Peroxide[J].Nature 1948,161,131-132) Catalase has been widely used in the analysis and detection of various targets due to its strong catalase activity. (Zhu, Z.; Guan, Z.; Jia, S.; Lei, Z.; Lin, S.; Zhang, H.; Ma, Y.; Tian, ​​Z.-Q.; Yang, C.J.Au@Pt Nanoparticle Encapsulated Target-Responsive Hydrogel with Volumetric Bar-Chart Chip Readout for Quantitative Point-of-Care Testing[J].Angew.Chem.,Int.Ed.2014,53,12503-12507;Song,Y.;Zhang,Y. ; Bernard, P.E.; Reuben, J.M.; Ueno...

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Problems solved by technology

(Zhu, Z.; Guan, Z.; Jia, S.; Lei, Z.; Lin, S.; Zhang, H.; Ma, Y.; Tian, ​​Z.-Q.; Yang, C.J.Au@Pt Nanoparticle Encapsulated Target-Responsive Hydrogel with Volumetric Bar-Chart Chip Readout for Quantitative Point-of-Care Testing[J].Angew.Chem.,Int.Ed.2014,53,12503-12507;Song,Y.;Zhang,Y. ; Bernard, P.E.; Reuben, J.M.; Ueno, N.T.; Arlinghaus, R.B.; 1-9; Song, Y.; Xia, X.; Wu, X.; Wang, P.; Qin, L. Integration of Platinum Nanoparticles with a Volumetric Bar-Chart Chip for Biomarker Assays[J].Angew.Chem., Int.Ed.2014,53,12451-12455; Song,Y.;Wang,Y.;Qin,L.A Multistage Volumetric BarChart Chip for Visualized Quantification of DNA[J].J.Am.Chem.Soc.2013,135, 16785-16788) However, catalase also has some shortcomings: first, as a protein, catalase is easily degraded in complex systems such as cell lysate or serum; secondly, the secondary structure of catalase Susceptible to extreme environments such as high temperature, strong acid and strong alkali, etc., which will cause irreversible decline in catalytic efficiency; (Song, Y.; Wang, Y.; Qin, L. A Multistage Volumetric Bar Chart Chip for Visualized Quantification of DNA[ J]. J. Am. Chem. Soc. 2013, 135, 16785-16788; Melov, S.; Ravenscroft, J.; Malik, S.; .S.; Walker, D.W.; Clayton, P.E.; Wallace, D.C.; Malfroy, B.; Doctrow, S.R.; Lithgow, G.J.Extension of Life-Span with Superoxide Dismutase / Catalase Mimetics[J]. 1569) Finally, the use of bioengineering to prepare, purify and preserve catalase keeps it relatively high in purity and catalytic activity.

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