A small peptide and composition formed by linking the small peptide

A technology of composition and linking carrier, which is applied in the direction of medical preparations, peptides, and pharmaceutical formulations of non-active ingredients, and can solve the problems of low plasmid transfection efficiency, affecting the development and application prospects of exosomes, and low recovery efficiency of exosomes

Active Publication Date: 2018-08-24
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of this method, such as low plasmid transfection efficiency, unclear expression conformation of targeted short peptide fusion expression, and low exosome recovery efficiency, it seriously affects the development and application prospects of exosome as a transport carrier.

Method used

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  • A small peptide and composition formed by linking the small peptide
  • A small peptide and composition formed by linking the small peptide
  • A small peptide and composition formed by linking the small peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Delivery composition formed by connecting exosome and targeting peptide by small peptide

[0065] The implementation process of this embodiment is as follows:

[0066] 1) In vitro recombinant exosome surface CD63 loop region, using the recombinant loop region as a substrate to screen the phage peptide library to obtain small peptides that specifically bind to the CD63 loop region;

[0067] 2) Combine the obtained small peptide with the targeting peptide in a coupling manner, and the combined chimeric short peptide is connected to the loop region of CD63 on the surface of the exosome by co-incubation with the exosome;

[0068] 3) Load the antisense oligonucleotide drug MOE-RNA into the exosome using the electroporation transformation method, and study the application of the exosome modified by the above targeting as a drug carrier.

[0069] Experimental part:

[0070] 1. Screening of small peptides specifically binding to CD63

[0071] The experimental steps are as follow...

Embodiment 2

[0194] Example 2 A composition with immune-promoting effect formed by connecting exosome and NIND with small peptide

[0195] The implementation process of this embodiment is as follows:

[0196] 1) The loop region of CD63 on the surface of the exosome was reorganized in vitro, and the recombinant loop region was used as a substrate to screen the phage peptide library to obtain the small peptide CP05 that specifically binds to the loop region of CD63;

[0197] 2) Coupling the obtained CP05 with the functional short peptide N1ND of the alarm element molecule (HMGN1), and the combined chimeric short peptide is connected to the loop region of CD63 on the surface of the exosome by co-incubation with the exosome.

[0198] Experimental part:

[0199] 1. Screening of small peptides specifically binding to CD63

[0200] This part is the same as the corresponding part of Embodiment 1, and will not be repeated.

[0201] 2. In vitro verification of small peptides

[0202] This part is the same as t...

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Abstract

The invention relates to the technical field of biological medicine projects, in particular to a small peptide and a screening method and application thereof. According to the application, the invention relates to a delivery composition composed of a small peptide connection carrier portion and a targeting peptide and a composition, with the function of promoting the immunity, composed of the small peptide connection carrier portion and a short peptide with the function of promoting the immunity.

Description

Technical field [0001] The present invention relates to the technical field of biomedical engineering, in particular to a small peptide, and its screening method and application. In terms of application, it relates to a delivery composition composed of a small peptide connected to a carrier part and a targeting peptide and a small peptide connected to a carrier Part and short peptides that promote immune function are composed of immune function-promoting compositions. Background technique [0002] Exosome is a biological nanovesicle structure secreted by cells and body fluids, with a diameter of about 30-100nm. It can carry endogenous mRNA, miRNA, and protein. It is a natural cellular messenger in the organism and can mediate signal transduction between cells. , The exchange of cellular materials and the presentation of antigens in cells and other biological processes. Recent studies have shown that exosomes have the characteristics of carrying biological information of donor ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K47/62
Inventor 尹海芳高先军左冰峰冉宁董雪
Owner TIANJIN MEDICAL UNIV
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