Longan pulp polysaccharide with enhanced functional activity and its green modification preparation method based on endogenous protein binding

A technology of longan pulp polysaccharide and functional activity is applied in the field of longan pulp polysaccharide and its green modification preparation based on the binding of endogenous proteins, and can solve the problems of weak biological activity of longan pulp polysaccharide, inability to meet development and utilization, residual toxic and harmful reagents, etc. , to avoid white matter co-precipitation, easy operation, and obvious effect of synergy

Active Publication Date: 2019-03-29
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) The biological activity of longan pulp polysaccharides is generally weak, and the high activity components are usually screened and separated by the activity tracking method, but the content of high activity components is low, which cannot meet the needs of development and utilization
[0007] (2) Although traditional chemical modification methods such as carboxymethylation, acetylation, and sulfation can enhance the biological activity of longan pulp polysaccharides, there are potential safety hazards of toxic and harmful reagent residues
The application of the Maillard reaction between polysaccharides and proteins in the improvement of protein structure and functional properties is in the ascendant, but the effect on the structure and biological activity of polysaccharides is rarely reported
There is no report on the protein-binding modification method and utility of longan pulp polysaccharides

Method used

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  • Longan pulp polysaccharide with enhanced functional activity and its green modification preparation method based on endogenous protein binding
  • Longan pulp polysaccharide with enhanced functional activity and its green modification preparation method based on endogenous protein binding
  • Longan pulp polysaccharide with enhanced functional activity and its green modification preparation method based on endogenous protein binding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of longan pulp polysaccharide with enhanced functional activity, the process is as follows figure 1 shown.

[0036]Step 1): Take fresh longan pulp, add distilled water of 3 times the mass, and homogenize intermittently at 11000r / min at high speed for 3 times, each time for 3 minutes; add 6 times the mass of distilled water to the homogenate, and adjust the pH value 8.5, stirring and leaching at room temperature for 4 hours; after the leaching, centrifuge at 4500r / min for 10min, filter and separate the supernatant A, adjust its pH to 5, and after standing for 3h, centrifuge at 4500r / min for 10min to separate the precipitate A and the above The supernatant B and the precipitate A were washed with distilled water with a pH value of 5, and then freeze-dried to obtain the longan pulp protein.

[0037] Step 2): Take the supernatant B obtained in step 1) and adjust the pH to be neutral, concentrate in a vacuum to 1 / 4 volume, add ethanol to a volume concentration o...

Embodiment 2

[0040] Preparation of longan pulp polysaccharide with enhanced functional activity

[0041] Step 1): Take the fresh longan pulp, add distilled water of 2 times the mass, and homogenize twice at a high speed intermittently at 12000r / min for 4 minutes each time; add 5 times the mass of distilled water to the homogenate, and adjust the pH value 9. Stir and extract at room temperature for 3 hours; after the extraction, centrifuge at 5000r / min for 8min, filter and separate the supernatant A, adjust its pH value to 5.5, and after standing for 2h, centrifuge at 5000r / min for 8min to separate the precipitate A and the above The supernatant B and the precipitate A were washed with distilled water with a pH value of 5.5, and then freeze-dried to obtain the longan pulp protein.

[0042] Step 2): Take the supernatant B obtained in step 1) and adjust the pH to be neutral. After vacuum concentration to 1 / 3 volume, add ethanol to a volume concentration of 80%, and place it at 3°C ​​for 14 ho...

Embodiment 3

[0045] Preparation of longan pulp polysaccharide with enhanced functional activity

[0046] Step 1): Take fresh longan pulp, add distilled water of 4 times the mass, and homogenize intermittently at 10,000 r / min at high speed for 4 times, each time for 2 minutes; add distilled water of 7 times the mass to the homogenate, and adjust the pH 8. Stir and extract at room temperature for 5 hours; after the extraction, centrifuge at 4000r / min for 12min, filter and separate the supernatant A, adjust its pH value to 4.5, and after standing for 4h, centrifuge at 4000r / min for 12min to separate the precipitate A and the above The supernatant B and the precipitate A were washed with distilled water with a pH value of 4.5, and then freeze-dried to obtain the longan pulp protein.

[0047] Step 2): Take the supernatant B obtained in step 1) and adjust the pH to be neutral, concentrate in vacuo to 1 / 5 volume, add ethanol to a volume concentration of 70%, and place it at 5°C for 10 hours to pr...

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Abstract

The invention belongs to the technical field of functional food processing, and specifically relates to a longan pulp polysaccharide with enhanced functional activity and a preparation method thereof based on intrinsic protein binding by green modification. The method comprises the following steps: 1) separating fresh longan pulp by adopting an alkali-extraction and acid-precipitation method so as to prepare longan pulp protein, and remaining an extraction supernatant from separated protein; 2) subjecting the extraction supernatant obtained by protein precipitation in the step 1) to alcohol precipitation so as to prepare longan pulp polysaccharide; and 3) inducing the longan pulp protein obtained in the step 1) and longan pulp polysaccharide obtained in the step 2) to covalent binding by a Maillard reaction so as to obtain the longan pulp polysaccharide with enhanced functional activity and based on intrinsic protein binding. The modified polysaccharide obtained by using the method provided by the invention has significantly reinforced antioxidant activity, immunoregulatory activity and antitumor activity compared with polysaccharide or protein before modification.

Description

technical field [0001] The invention belongs to the technical field of functional food processing, and in particular relates to a longan pulp polysaccharide with enhanced functional activity and a green modification preparation method based on endogenous protein binding. Background technique [0002] Longan (Dimocarpus longan Lour.) is an economic crop of Sapindaceae. It is mainly distributed in Guangdong, Guangxi, Fujian and Hainan provinces in my country, and its planting area and output are much larger than other major producing countries. Longan pulp is a medicinal and edible fruit included in the Pharmacopoeia. It not only has a unique flavor and is rich in nutrition, it can also treat / improve symptoms such as insufficient Qi and blood, palpitations, insomnia, forgetfulness, anemia, spleen deficiency and diarrhea. In the new "value-driven" food consumption Under normal circumstances, it is deeply loved by consumers. The harvest period of longan fruit is short, the mark...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08H1/00
CPCC08B37/0003C08B37/006C08H1/00
Inventor 易阳王宏勋王丽梅闵婷侯温甫艾有伟
Owner WUHAN POLYTECHNIC UNIVERSITY
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