A preparation of megakaryotic progenitor cells and its application and preparation method

A technology of megakaryotic progenitor cells and preparations, applied in the field of stem cell preparations, can solve problems such as prone to adverse reactions and unsatisfactory treatment effects

Active Publication Date: 2019-02-19
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the therapeutic effect of the injection is not satisfactory, and adverse reactions are prone to occur

Method used

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  • A preparation of megakaryotic progenitor cells and its application and preparation method
  • A preparation of megakaryotic progenitor cells and its application and preparation method
  • A preparation of megakaryotic progenitor cells and its application and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1 Preparation of megakaryotic progenitor cells

[0080] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.

[0081] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.

[0082] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 2mL human serum albumin, 1.5mL stem cell growth factor, 0.5mL interleukin 2 and 11mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.

[0083] This megakaryotic progenitor cell preparation includes:

[0084]

Embodiment 2

[0085] Example 2 Preparation of megakaryotic progenitor cells

[0086] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.

[0087] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.

[0088] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 3mL human serum albumin, 1mL stem cell growth factor, 1mL interleukin 2 and 10mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.

[0089] This megakaryotic progenitor cell preparation includes:

[0090]

[0091]

Embodiment 3

[0092] Example 3 Preparation of megakaryotic progenitor cells

[0093] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.

[0094] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.

[0095] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 4mL human serum albumin, 0.5mL stem cell growth factor, 1.5mL interleukin 2 and 9mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.

[0096] This megakaryotic progenitor cell preparation includes:

[0097]

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Abstract

The invention relates to the technical field of a stem cell preparation, in particular to a megakaryocyte progenitor cell preparation as well as application and a preparation method thereof. The megakaryocyte progenitor cell preparation consists of megakaryocyte progenitor cells, human albumin, a stem cell growth factor, interleukin-2 and a safflower injection. The megakaryocyte progenitor cell preparation disclosed by the invention has a significant effect on improving aleucia; after infusion, platelet content in patients, as being recovered, can be kept at a normal level for a relatively long time in vivo; and the megakaryocyte progenitor cell preparation, after being applied, is free from any adverse reaction and safe to use, and the megakaryocyte progenitor cell preparation is simple in preparation and convenient to use.

Description

technical field [0001] The invention relates to the technical field of stem cell preparations, in particular to a preparation of megakaryocyte progenitor cells and its application and preparation method. Background technique [0002] A sufficient number of platelets with normal function is an indispensable part of the hemostasis process, and the normal value of platelets is about 150-450×10 3 / mm 3 , so broadly speaking, less than 150,000 can be called thrombocytopenia (Thrombocytopenia). In foreign countries, umbilical cord blood was directly taken from 5600-9100 fetuses to test the platelet count, and it was found that the proportion of neonatal platelet deficiency was about 0.7-0.9% (Sola et al., 2000). Thrombocytopenia is also the most common bleeding disorder in the neonatal intensive care unit (NICU). Today, hematopoietic stem cell transplantation has been widely used in the clinical treatment of malignant and non-malignant blood diseases, solid tumors, genetic meta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/38A61P7/00A61K35/28A61K36/286A61K38/18A61K38/20
Inventor 葛啸虎陈海佳王一飞曾维杰李平
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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