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Morinda citrifolia extract and application thereof to preparation of drugs for controlling plant anthracnose

A technology of extract and noni fruit, applied in noni fruit extract and its application in the preparation of medicines for preventing and treating plant anthracnose, plant active extract and its application field, achieving the effect of simple preparation method and low cost

Inactive Publication Date: 2015-12-16
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After retrieval, there is no report on the application of noni fruit extract in the prevention and treatment of plant anthracnose

Method used

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  • Morinda citrifolia extract and application thereof to preparation of drugs for controlling plant anthracnose

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Squeeze fresh ripe noni fruit juice, filter it with 4 layers of gauze, centrifuge at 4000r / min for 20min, and the supernatant is noni juice. Noni juice was freeze-dried into powder by freeze dryer, leached 3 times with 95% ethanol ultrasonic (ultrasonic frequency 40kHz, temperature 30°C, time 20min), combined the extracts for suction filtration, and the filtrate was passed through a vacuum rotary evaporator (Water bath temperature 45 ℃) concentrated into extractum to obtain Noni fruit ethanol extract.

Embodiment 2

[0015] Squeeze fresh ripe noni fruit juice, filter it with 4 layers of gauze, centrifuge it at 5000r / min for 15min, and the supernatant is noni juice. Noni juice was freeze-dried into powder by freeze dryer, leached three times with 95% ethanol ultrasonic (ultrasonic frequency 40kHz, temperature 32°C, time 30min), combined the extracts for suction filtration, and the filtrate was passed through a vacuum rotary evaporator (Water bath temperature 45 ℃) concentrated into extractum to obtain Noni fruit ethanol extract. Add 2 times the volume of noni ethanol extract in distilled water to make a suspension, extract with an equal volume of petroleum ether (boiling range 60-90°C) of the suspension, and concentrate the extraction phase with a vacuum rotary evaporator to obtain noni petroleum ether site extract.

Embodiment 3

[0017] Squeeze fresh ripe noni fruit juice, filter it with 4 layers of gauze, centrifuge it at 5000r / min for 10min, and the supernatant is noni juice. Noni juice was freeze-dried into powder by freeze dryer, leached three times with 95% ethanol ultrasonic (ultrasonic frequency 40kHz, temperature 34°C, time 40min), combined the extracts for suction filtration, and the filtrate was passed through a vacuum rotary evaporator (Water bath temperature 45 ℃) concentrated into extractum to obtain Noni fruit ethanol extract. Add 2 times the volume of noni ethanol extract in distilled water to make a suspension, extract with ethyl acetate equal to the volume of the suspension, and concentrate the extract phase by a vacuum rotary evaporator to obtain the extract of noni ethyl acetate.

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Abstract

The invention belongs to the field of plant applications and relates to a morinda citrifolia extract and an application thereof to preparation of drugs for controlling plant anthracnose. Fresh and mature morinda citrifolia is juiced, the juiced morinda citrifolia is filtered and centrifuged, morinda citrifolia juice is obtained and freeze-dried into powder, the powder is subjected to ultrasonic extraction with ethanol, an extract liquid is subjected to suction filtration, a filtrate is concentrated into an extract, and the morinda citrifolia extract is obtained. On the basis of a bacteriostasis experiment, the morinda citrifolia extract is found to have good efficacy on control of the plant anthracnose and can be used for preparing the drugs for controlling the plant anthracnose, an effective way is provided for control of the plant anthracnose, and a preparation method is simple and low in cost.

Description

technical field [0001] The invention belongs to the field of plant application, and relates to a plant active extract and its application, in particular to a noni fruit extract and its application in the preparation of medicaments for preventing and treating plant anthracnose. Background technique [0002] Plant anthracnose is a general term for the diseases caused by the sexual generations of a class of fungi in the pathogenic bacteria, that is, the fungi of the genus Colletotrichum in the order Nigella. The disease mainly harms the leaves and fruits, and can also harm the shoots, causing leaf spots, defoliation, fruit rot and dead shoots. The main feature of the disease is that the diseased part is easy to produce black dots, which are often in the shape of rings, and pink sticky spore clusters overflow under humid conditions. At present, the main method of preventing and controlling plant anthracnose is chemical control. Although the control effect is better, chemical co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P3/00
Inventor 谭琳臧小平马伏宁刘以道郑晓燕马蔚红
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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