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Application of polysaccharide components of Inonotus obliquus in the prevention and treatment of chronic pancreatitis

A technology for chronic pancreatitis and Inonotus obliquus, which is applied in the field of medicine to achieve the effects of reducing the content, reducing the production of IL-1β and IFN-γ, and slowing down immune metabolism

Inactive Publication Date: 2017-11-28
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the study of polysaccharides from Inonotus obliquus in slowing down chronic pancreatitis

Method used

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  • Application of polysaccharide components of Inonotus obliquus in the prevention and treatment of chronic pancreatitis
  • Application of polysaccharide components of Inonotus obliquus in the prevention and treatment of chronic pancreatitis
  • Application of polysaccharide components of Inonotus obliquus in the prevention and treatment of chronic pancreatitis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The extraction, purification and analysis of the polysaccharide component of embodiment 1 Inonotus obliquus

[0035] The sclerotium of Inonotus obliquus was provided by Baikaltai Bioengineering Company, Harbin, China. Glucose (Glu), galactose (Gal), rhamnose (Rha), arabinose (Ara), xylose (Xyl), galacturonic acid (GalU A), purchased from sigma company; derivatization reagent: 1- Phenyl-3-methyl-5-pyrazolone (PMP), with a purity of 99%, was purchased from Across Organics.

[0036] High performance liquid chromatography (Agilent 1200 type), Agilent Technologies Co., Ltd.; chromatographic column: HypersilBDS-C18 (250mm×4.6mm, 5μm), Dalian Elite Analytical Instrument Co., Ltd.

[0037] (1) Extraction of Inonotus obliquus polysaccharide components

[0038] The sclerotium of Inonotus obliquus needs to be dried in an oven at 70°C to constant weight, then ground into powder, added with 70% to 80% ethanol by volume, and extracted at room temperature for 24-72 hours to remove i...

Embodiment 2

[0054] The pharmacological activity evaluation of embodiment 2 Inonotus obliquus polysaccharide components

[0055] (1) Test material:

[0056] The mice used in the experiment were five-week-old Kunming white mice purchased from the Drug Safety Evaluation Center of Heilongjiang University of Traditional Chinese Medicine. Sodium diethyldithiocarbamate was purchased from Jining Huide Chemical Co., Ltd., Qingyi Lidan Granules were purchased from Changchun Yinnuoke Pharmaceutical Co., Ltd., cytokine ELISA kit, amylase and lactate dehydrogenase kit and hydroxyl The proline kit was purchased from Nanjing Jiancheng Technology Co., Ltd., and the polysaccharide component of Inonotus obliquus was the 10th tube of IOP-1 prepared according to the method in Example 1, that is, IOP-1 (10).

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Abstract

The invention discloses application of Inonotus obliquus polysaccharide components in preventing and controlling chronic pancreatitis and belongs to the technical field of medicaments. The Inonotus obliquus polysaccharide components are applicable to preventing and controlling chronic pancreatitis. The Inonotus obliquus polysaccharide components which are 0.4g / kg of mouse weight are effective in increasing the content of superoxide dismutase in pancreatic glands of mice with chronic pancreatitis and decreasing accumulation of malondialdehyde and meanwhile are capable of reducing IL-1Beta and IFN-Nu in serum, and effective in decreasing the contents of amylase and lactic dehydrogenase and decreasing synthetic hydroxyproline. An application method of Inonotus obliquus polysaccharide components alleviate the condition of chronic pancreatitis in terms of immunity and metabolic regulation; compared with methods such as accumulated heat and dyspepsia conditioning in traditional Chinese medicine and medicated analgesia and surgery in western medicine, the method has the advantages that no toxic or side effect occurs, surgical pain is avoided, a treatment course is short, treatment is safe and convenient, and the effect of preventing and treating chronic pancreatitis in mice is significant.

Description

technical field [0001] The invention relates to a natural edible and medicinal fungus polysaccharide component, in particular to the application of the polysaccharide component of Inonotus obliquus in preventing and treating chronic pancreatitis, and belongs to the technical field of medicine. Background technique [0002] Chronic pancreatitis is a long-standing pancreas inflammation that can change the normal structure of the tissue and the endocrine function. Chronic pancreatitis is characterized by inflammatory cell infiltration, acinar atrophy, granulation tissue formation, irregular fibrosis, and ductal abnormalities, among others (Inui,K.,Yoshino,J.,Miyoshi,H.,Yamamoto,S.,Kobayashi, T., 2013. New developments in diagnosis and non-surgical treatment of chronic pancreatitis. Journal of Gastroenterology and Hepatology. 28, 108–112.). Pancreatic fibrosis is a typical histopathological feature of the pancreas (Phillips P., 2012. Pancreatic stellate cells and fibrosis, in: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P1/18A61P29/00C08B37/00
Inventor 曲娟娟赵伟胡洋黎扣扣
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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