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Quality detection method of experimental primate animal sperms

A primate and sperm quality technology, applied in the field of primate laboratory animal sperm quality detection, can solve the problems of low success rate, affecting other index detection, difficulty in primate laboratory animal semen collection and the like

Inactive Publication Date: 2015-12-16
CHINA AGRI UNIV
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Problems solved by technology

[0002] The current common sperm quality analysis methods are only applicable to human semen, and it is difficult to collect semen from primate experimental animals. The commonly used method of collecting semen by electrical stimulation is likely to cause animal tissue damage and affect the detection of other indicators
In addition, electric stimulation takes a long time to collect semen, the success rate is low, and it cannot meet the sperm quality analysis requirements of primate experimental animals

Method used

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  • Quality detection method of experimental primate animal sperms
  • Quality detection method of experimental primate animal sperms
  • Quality detection method of experimental primate animal sperms

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Embodiment 1

[0023] Embodiment 1, primate experimental animal sperm quality detection method

[0024] 1. Preparation of sperm capacitation solution

[0025] The sperm capacitation solution was prepared as follows: NaCl, KCl, MgCl 2 , CaCl 2 , NaH 2 PO 4 , NaHCO 3 , glucose, sodium pyruvate, sodium lactate, Hepes (4-hydroxyethylpiperazineethanesulfonic acid), BSA, gentamicin sulfate and pure water are mixed to obtain sperm capacitation fluid, wherein the final concentration of each solute is as follows: NaCl114mM, KCl3.2mM, MgCl 2 0.5mM, CaCl 2 2.0mM,NaH 2 PO 4 0.4mM, NaHCO 3 24.9mM, glucose 5.6mM, sodium pyruvate 0.5mM, sodium lactate 10mM, Hepes 10mM, BSA 3g / L, gentamicin sulfate 30mg / L.

[0026] 2. Testing methods for the sperm quality of primate experimental animals

[0027] The selective mature cynomolgus monkey was used as the experimental object, and the standard evaluation was based on the fifth edition of WHO's "Laboratory Manual for Examination and Processing of Human S...

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Abstract

The invention discloses a quality detection method of experimental primate animal sperms and provides a quality detection kit of primate animal sperms. The quality detection kit of primate animal sperms comprises a sperm capacitation solution. A method for preparing the sperm capacitation solution comprises the step of mixing NaCl, KCl, MgCl2, CaCl2, NaH2PO4, NaHCO3, glucose, sodium pyruvate, sodium lactate and Hepes with water to obtain the sperm capacitation solution, wherein the concentration of NaCl is 114mM; the concentration of Cl is 3.2mM; the concentration of MgCl2 is 0.5mM; the concentration of CaCl2 is 2.0mM; the concentration of NaH2PO4 is 0.4mM; the concentration of NaHCO3 is 24.9mM; the concentration of the glucose is 5.6mM; the concentration of sodium pyruvate is 0.5mM; the concentration of sodium lactate is 10mM; the concentration of Hepes is 10mM. By virtue of the method, the damage to the reproductive system caused by collecting seminal fluid through electrical stimulation can be avoided; meanwhile, the method is capable of quickly detecting the quality of the sperms while sampling experimental animals, so that the method has a broad application prospect in research such as food safety evaluation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting the sperm quality of primate experimental animals. Background technique [0002] The current common sperm quality analysis methods are only applicable to human semen, and it is difficult to collect semen from primate experimental animals. The commonly used method of collecting semen by electrical stimulation is likely to cause animal tissue damage and affect the detection of other indicators. In addition, electrical stimulation takes a long time to collect semen, and the success rate is low, which cannot meet the requirements for sperm quality analysis of primate experimental animals. [0003] Sperm motility analysis is the most commonly used examination method to evaluate male fertility. Computer-assisted sperm analysis (computer-aidedspermanalysis, CASA) applies computer technology and advanced image processing technology to sperm quality analysis, and provid...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
Inventor 崔胜潘吉荣张丽刘佳利
Owner CHINA AGRI UNIV
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