Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bivalent propolis inactivated vaccine for rabbit hemorrhagic disease and multocida pasteurellosis and preparation method of bivalent propolis inactivated vaccine

A rabbit virus hemorrhage, Pasteurella technology, applied in the direction of antiviral agents, viral antigen components, antibacterial drugs, etc., can solve the problem of low immune protection rate of Pasteurellosis multocida, and achieve excellent immune protection rate , the effect of reducing stress

Inactive Publication Date: 2015-12-23
山东华宏生物工程有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the patent document CN101732704A discloses a preparation method of rabbit viral hemorrhagic disease and pasteurellosis multocida dual inactivated vaccine. In this patent of invention, preservative thimerosal is added to increase the immune protection rate of pasteurellosis multocida slightly lower

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bivalent propolis inactivated vaccine for rabbit hemorrhagic disease and multocida pasteurellosis and preparation method of bivalent propolis inactivated vaccine
  • Bivalent propolis inactivated vaccine for rabbit hemorrhagic disease and multocida pasteurellosis and preparation method of bivalent propolis inactivated vaccine
  • Bivalent propolis inactivated vaccine for rabbit hemorrhagic disease and multocida pasteurellosis and preparation method of bivalent propolis inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 2

[0032] The preparation of embodiment 1 double propolis vaccine

[0033] 1. Preparation of tissue suspension for rabbit viral hemorrhagic disease seedling preparation

[0034] For inoculation, take the virus seed for production, dilute it 10 times with inactivated normal saline, and inject 1 mL subcutaneously in the neck of each production test rabbit. Harvest the liver tissues of dying or just dead rabbits 24-96 hours after inoculation under aseptic conditions, and conduct pathological examination one by one. If there is abnormal liver disease, the liver should be discarded. After collecting the poison, it should be used for seedling preparation or frozen quickly. Store at -10°C for no more than 15 days; store below -20°C for no more than 50 days. Under sterile conditions, the harvested liver tissue was weighed, mashed with an appropriate amount of normal saline, and then ground by a colloid mill. The final ratio of tissue to diluent was 1:4. Add formaldehyde solution accor...

Embodiment 2 2

[0043] The sterility test of embodiment 2 double propolis vaccines

[0044] Before, during and after subpackaging, 5 vial samples of each batch of dual vaccine were randomly taken, and the sterility test was carried out according to the appendix 448 of the "Regulations of the People's Republic of China for Veterinary Biological Products", and the results showed that all of them grew aseptically.

Embodiment 3 2

[0045] The safety inspection of embodiment 3 double propolis vaccines

[0046] Randomly select 5 vials of vaccine samples from each batch, mix the samples from each batch, and take samples to subcutaneously inject 4 experimental rabbits of about 1.5kg, each subcutaneously injected with 4.0mL, and observe for 10 days, all of which are healthy and alive.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of animal vaccine preparation and particularly relates to a bivalent propolis inactivated vaccine for rabbit hemorrhagic disease and multocida pasteurellosis and a preparation method of the bivalent propolis inactivated vaccine. The preparation of the bivalent propolis inactivated vaccine is finished by using a rabbit hemorrhagic disease pathological tissue suspension and a multocida pasteurellosis liquid antigen through the steps of preparing a rabbit hemorrhagic disease production virus seed; preparing the rabbit hemorrhagic disease pathological tissue suspension; preparing a multocida pasteurellosis production seed; preparing a multocida pasteurellosis vaccine preparation liquid; preparing a propolis ethanol leachate; and preparing the bivalent propolis inactivated vaccine. The bivalent propolis inactivated vaccine disclosed by the invention has the immune protective rate of 100% for the rabbit hemorrhagic disease and the immune protective rate of 90% for the multocida pasteurellosis and is long in storage period; and meanwhile, due to the combination of the vaccine, the immune procedure is simplified, the labor is saved, and the immune cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of animal vaccine preparation, and in particular relates to a dual propolis inactivated vaccine for rabbit viral hemorrhagic disease and pasteurellosis multocida and a preparation method thereof. Background technique [0002] Rabbit viral hemorrhagic disease, also known as rabbit plague, is an acute and highly contagious infectious disease of rabbits caused by rabbit viral hemorrhagic disease virus. It is characterized by respiratory system hemorrhage, parenchymal organ edema, congestion, and bleeding changes. The disease was first discovered in Jiangsu Province of my country in the spring of 1984, and spread rapidly to the whole country, and then occurred all over the world. The disease is often fulminant, with extremely high morbidity and mortality, which has brought great harm to the rabbit industry in the world. It is one of the main infectious diseases in large-scale rabbit farms. [0003] Pasteurellos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K39/102A61K39/12A61K39/39A61P31/04A61P31/12
Inventor 王晓丽王永明张效伟朱万光李晓霞
Owner 山东华宏生物工程有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com