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Quinoa protein and extraction method thereof

A technology of quinoa protein and extraction method, applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve the problems of blank quinoa protein extraction technology, improve immunity, promote digestion, and achieve high purity Effect

Inactive Publication Date: 2015-12-23
QINGHAI TSINGHUA BIOTRY BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no specific record about the specific extraction process for quinoa protein, so the extraction technology of quinoa protein is still in a blank state

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The extraction method of quinoa protein is as follows:

[0027] 1) Wash the quinoa with clean water, and remove impurities, select high-quality quinoa, soak the quinoa in clean water for 4 hours to soften it and facilitate grinding, then soak the quinoa with 50 mesh Filter through a sieve, dry and let stand for 30 minutes;

[0028] 2) Grind the quinoa into a powder with a mesh size of 120 or more, using an ordinary grinder; add NaOH to the quinoa powder (the mass ratio of quinoa / NaOH solution is 10:1, and the concentration of NaOH solution is 0.015mol / L). Then it was stirred at room temperature for 2 h (stirring rate was 200 rad / min). Store the mixed and stirred quinoa puree in a refrigerator at 4°C for 24 hours;

[0029] 3) Centrifuge the quinoa puree preserved at low temperature at 10°C (speed 8000rad / min, processing time 30min); take the supernatant, filter it with a 100-mesh sieve, and add HCL with a concentration of 0.8mol / L solution, adjust the acidity of the f...

Embodiment 2

[0033] The extraction method of quinoa protein is as follows:

[0034] 1) Wash the quinoa with clean water and remove impurities, select high-quality quinoa, soak the quinoa in clean water for 5 hours to soften it and facilitate grinding, then soak the quinoa with 60 mesh Filter through a sieve, dry and let stand for 40 minutes;

[0035]2) Grind the quinoa into a powder with a mesh size of 120 or more, using an ordinary grinder; add NaOH to the quinoa flour (the mass ratio of quinoa / NaOH solution is 10:2, and the concentration of NaOH solution is 0.02mol / L). Then it was stirred at room temperature for 3 h (stirring rate was 300 rad / min). Store the mixed and stirred quinoa puree in a refrigerator at 5°C for 25 hours;

[0036] 3) Centrifuge the quinoa puree preserved at low temperature at 15°C (speed 9000rad / min, processing time 40min); take the supernatant, filter it with a 100-mesh sieve, and add HCL with a concentration of 1.5mol / L solution, adjust the acidity of the filtr...

Embodiment 3

[0040] The extraction method of quinoa protein is as follows:

[0041] 1) Wash the quinoa with clean water, and remove impurities, select high-quality quinoa, soak the quinoa in clean water for 4.5 hours to soften it and facilitate grinding, and then soak the quinoa with 50 Filter through a sieve above mesh, dry and let stand for 30 minutes;

[0042] 2) Grind the quinoa into a powder with a mesh size of 120 or more, using an ordinary grinder; add NaOH to the quinoa flour (the mass ratio of quinoa / NaOH solution is 10:1.5, and the concentration of NaOH solution is 0.018mol / L). Then it was stirred at room temperature for 2.5 h (stirring rate was 250 rad / min). Store the mixed and stirred quinoa puree in a refrigerator at 5°C for 25 hours;

[0043] 3) Centrifuge the quinoa puree preserved at low temperature at 10°C (speed 8500rad / min, processing time 30min); take the supernatant, filter it with a 100-mesh sieve, and add HCL with a concentration of 0.9mol / L Solution, adjust the a...

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Abstract

The invention provides a quinoa protein and an extraction method thereof. The extraction method comprises the following steps: (A) cleaning quinoa, soaking the quinoa for a period of time, pulverizing the quinoa, adding a NaOH solution, performing stirring for a period of time, and performing preservation at low temperature for more than 24h to obtain quinoa paste; (B) centrifugally treating the quinoa paste to obtain a supernatant, and adjusting the pH to 4-5 so as to obtain a protein precipitation; (C) cleaning, centrifugally separating and freeze-drying the protein precipitation to obtain the quinoa protein. The extraction method of the quinoa protein, provided by the embodiment of the invention, is simple and easy to operate, and operation conditions are mild; a referable method is provided for a subsequent quinoa protein extraction process, and the method has great reference value, and the obtained quinoa protein is high in purity.

Description

technical field [0001] The invention relates to the field of protein extraction and processing, in particular to a quinoa protein and an extraction method thereof. Background technique [0002] Quinoa (scientific name: Chenopodiumquinoawilld) is native to the Andes in South America. It is the main traditional food of the Inca indigenous people. It has a planting history of more than 5,000-7,000 years. Because of its unique rich and comprehensive nutritional value, it has nurtured the Inca nation. The ancient Incas called it "the mother of food". The colors of quinoa seeds are mainly white, black, and red, and the nutritional content is not much different. Among them, the white taste is the best, while the black and red seeds are relatively poor in taste and the seeds are also small. Quinoa was used by NASA as space food for astronauts in the 1980s. The Food and Agriculture Organization of the United Nations believes that quinoa is the only food that can basically meet the ...

Claims

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Application Information

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IPC IPC(8): C07K1/30
Inventor 董轲龚晨陈小波张一声李婷于雪安月玲
Owner QINGHAI TSINGHUA BIOTRY BIO TECH
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