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Cultivation of Rhodopseudomonas and its long-term survival method using red soil

A technique of Rhodopseudomonas and Rhodopseudomonas marsh is applied in the fields of using red soil to cultivate Rhodopseudomonas and its long-term preservation, cultivation of strains and its preservation, so as to prolong the survival, prolong the storage time, survival effect

Inactive Publication Date: 2019-04-02
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no relevant report on the cultivation and preservation of Rhodopseudomonas using red soil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 is used for the preparation of the culture material of embodiment 2~4 and the modification of laterite:

[0032] (1) Water treatment: Take fresh seawater with a salinity of 25~35‰ from the sea, fill it in a container, store it in the dark and dark for 60 to 90 days, and use it as old seawater for later use; pass potable water through a 0.45μm membrane Filter to get fresh water, set aside;

[0033] (2) Bagasse treatment: take fresh bagasse, dry and crush it, pass through a 80-100 mesh sieve, soak it in 95% alcohol for 24 hours to remove the alcohol liquid, then soak it in 75% alcohol for 24 hours to remove the alcohol liquid, and let it dry in sunlight Dried in the sun or dried at 90°C to obtain the processed bagasse for later use;

[0034] (3) Medium configuration: take NH 4 C1 1.0g, CH 3 COONa 3.5g, MgCl 2 0.1g, CaCl 2 0.1g, KH 2 PO 4 0.6g, K 2 HPO 4 0.4g, 0.1g of yeast extract to prepare the composition of Rhodopseudomonas conventional mediu...

Embodiment 2

[0038] Embodiment 2 Utilize laterite to cultivate Rhodopseudomonas, the steps are as follows:

[0039] (1) Inoculation culture: Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), with 3000 LX light, 34°C, and anaerobic culture for 3 days to complete the expanded culture of the strain; then with an inoculum size of 5 mL / 100mL, cultured for 3 days, and completed the second stage of expansion under the same light, temperature, and anaerobic conditions Cultivate; then culture with an inoculum size of 40mL / 100mL for 3 days, and complete productive culture under the same light, temperature and anaerobic conditions to obtain liquid Rhodopseudomonas;

[0040] (2) Cultivate laterite culture: After 2 days of productive cultivation, the color of the liquid turns deep red and the pH is 8.0-9.0, add 250g / L of cultured red soil until the pH of the upper liquid is 7.0-8.0, and continue anaerobic cultivation for 5 days , increase the concentration of Rhodopseudomonas until the cultur...

Embodiment 3

[0047] Embodiment 3 Utilize laterite to cultivate Rhodopseudomonas, the steps are as follows:

[0048] (1) Inoculation culture: Rhodopseudomonas palustris ( Rhodopseudomonas palustris ), with 3000LX light, 32°C, and anaerobic culture for 7 days to complete the expanded culture of the strain; then with an inoculum size of 3 mL / 100mL, cultured for 7 days, and completed the second level of expanded culture under the same light, temperature, and anaerobic conditions ; Then with the inoculum size 30mL / 100mL, cultivate 5d, complete the productive cultivation under the same light, temperature and anaerobic conditions, and obtain liquid Rhodopseudomonas;

[0049] (2) Cultivate laterite culture: After 5 days of productive cultivation, the color of the liquid turns deep red and the pH is 8.0~9.0, add 50g / L of cultured red soil until the pH of the upper layer liquid is 7.0~8.0, and continue anaerobic cultivation for 4d , increase the concentration of Rhodopseudomonas until the culture b...

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Abstract

The invention discloses a Rhodopseudomonas strain cultured by using red soil and a long-term preservation method thereof. The red soil is accessible and has the characteristics of low cost, high adsorptivity for nitrogen compounds and the like, low pH and high buffer capacity; and thus, after being modified and used for culturing the Rhodopseudomonas strain, the red soil can provide necessary nutritive salt slow release and pH buffering for strain growth, thereby effectively solving the problem of death of Rhodopseudomonas in the storage period. The Rhodopseudomonas product obtained by the method is a solid product, and has the advantages of long storage time and high Rhodopseudomonas palustris activity. The Rhodopseudomonas palustris content of the Rhodopseudomonas product is 10<9>-10<10> cfu / g, and the pH value is 7.0-8.0; the storage time of the Rhodopseudomonas product at normal temperature or room temperature under sealed vacuum conditions is 6-8 months; and the Rhodopseudomonas palustris activity is kept by 90-95%.

Description

technical field [0001] The invention relates to a method for cultivating and keeping alive strains, in particular to a method for cultivating Rhodopseudomonas by using red soil and keeping alive for a long time, belonging to the technical field of strain cultivation in the field of biotechnology. Background technique [0002] During the productive culture of the photosynthetic bacterium Rhodopseudomonas, the increasing pH of the culture solution is an obstacle to the increase of the culture concentration, and the accumulation of a large amount of metabolic waste is also a problem. Rhodopseudomonas products are generally packaged in liquid transparent cans and exposed to light. During sales and use, the shelf life or storage time is as long as 3 to 6 months. Rhodopseudomonas products are stored for a long period of time, where the survival of the bacteria is a problem. Due to the consumption of nutrients and the increase of metabolic waste, a large number of PSBs die (98%~10...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 邱明生邱德全于鸽杨世平胡蝶宋采
Owner GUANGDONG OCEAN UNIVERSITY
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