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A method for adjusting the acid-alcohol ratio in the fermentation product of anaerobic aerobic microorganisms

A microbial fermentation and product technology, applied in fermentation, biofuel and other directions, can solve the problems of complex fermentation characteristics, lack of fermentation product production control means, and limited application of anaerobic gas-eating microorganisms, and achieve the effect of improving technical value.

Active Publication Date: 2020-03-17
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation characteristics of such microorganisms are complex, and there is a lack of means for the purposeful production and regulation of fermentation products, which limits the application of anaerobic microorganisms.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Adjust the initial pH of the system to 6.0, the MES concentration is 5g / L, see the specific operation method for other conditions, the system is shaken and cultivated in a constant temperature shaker, and the samples are taken out at regular intervals to measure the acetic acid, ethanol and butanol in the fermentation broth by HPLC method Concentration detection. After 6 days of fermentation, the concentration of ethanol in the system was 0.34g / L, butanol was 0g / L, acetic acid was 1.47g / L, and the ratio of acid to alcohol was 4.3:1. Under the conditions of higher pH and MES concentration, organic The yield of acids is higher.

Embodiment 2

[0027] Adjust the initial pH of the system to 6.0, and the MES concentration to 0.1g / L. For other conditions, see the specific operation method. The system is shaken and cultivated in a constant temperature shaker. Samples are taken out at regular intervals and analyzed by HPLC. Detection of alcohol concentration. After 6 days of fermentation, the concentration of ethanol in the system was 0.11g / L, butanol was 0g / L, acetic acid was 1.79g / L, and the ratio of acid to alcohol was 16.3:1.

[0028] The results of Examples 1 and 2 show that increasing the initial pH of the system while reducing the amount of MES can significantly increase the proportion of organic acids in the fermentation product.

Embodiment 3

[0030] Adjust the initial pH of the system to 5.0, and the MES concentration is 0.1g / L. For other conditions, please refer to the specific operation method. The system is shaken and cultivated in a constant temperature shaker, and samples are taken out at regular intervals, and the acetic acid, ethanol and butyrate in the fermentation broth are tested by HPLC method. Detection of alcohol concentration. After 6 days of fermentation, the concentration of ethanol in the system was 0.51g / L, butanol was 0.065g / L, acetic acid was 0.12g / L, and the ratio of acid to alcohol was 0.21:1. In a slightly lower pH and low concentration MES system, alcohol The yield of the species is higher.

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PUM

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Abstract

The invention aims to provide a culture method for regulating acid and alcohol proportions in anaerobic gas-absorbing microorganism fermentation products. The method is capable of regulating the acid and alcohol proportions in the fermentation products by changing culture conditions. Particularly, the method is capable of regulating the alcohol substance proportion in the fermentation products in a shaking culture system by changing culture medium conditions. The method for increasing the alcohol substance proportion in the anaerobic gas-absorbing microorganism fermentation products is implemented by regulating an initial pH (potential of hydrogen) value and mes concentration of the system, wherein the high initial pH value and the low mes concentration can increase the acid substance proportion in the fermentation products remarkably; the slightly low initial pH value and the slightly low mes concentration can increase the alcohol substance proportion in the fermentation products remarkably.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for adjusting the acid-alcohol ratio in anaerobic microbial fermentation products. [0002] technical background [0003] As a major consumer of resources in the world, my country is facing the severe situation of gradual depletion of fossil resources and increasing carbon emissions. The excessive use of fossil resources such as oil and natural gas has brought about many social problems such as sustainable development and utilization of energy and chemicals, and environmental pollution. Hot Issues. Adjusting energy structure and developing renewable resources has become an inevitable choice for my country to achieve sustainable development. Carbon-containing gas refers to a kind of mixed gas with CO as the main component. Most of these gases are composed of CO, CO 2 and H 2 , is a kind of cheap raw material for the manufacture of petrochemica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12P7/06C12P7/62
CPCY02E50/10
Inventor 韩一凡郭亚琼谢彬涛王兴彪王敬敬张小霞黄志勇
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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