Method for extracting small-molecule humic acid from soil through XAD resin

A technology of humic acid and small molecules, applied in the preparation of test samples, etc., can solve the problems of lack, lack of knowledge of small molecule humic acids, etc., and achieve the effect of improving extraction efficiency

Inactive Publication Date: 2015-12-30
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the severe lack of samples, people

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1: the extraction of small molecule humic acid in the soil of East Xiaokou Forest Park, Changping District, Beijing:

[0058] H-type cationic resin: Bio-RadAG-MP-5;

[0059] NaOH: analytically pure; HCl: analytically pure;

[0060] The extraction steps are as follows:

[0061] Step a: soil treatment: take natural soil, remove impurities such as tree roots and stones, after air-drying, grind through a 100-mesh sieve to obtain a soil sample;

[0062] Step b: Sodium pyrophosphate extracts soil humic acid: Weigh 1Kg of the soil sample in step a and add 5L of deionized water to make the solid-to-liquid ratio 1:5, then adjust the pH of the solution to 1.0 with 6mol / LHCl and 6mol / LNaOH , then add 0.1mol / L HCl to it to a solid-liquid ratio of 1:10, stir the solution continuously for 4 hours, then let it stand for 24 hours, and centrifuge to obtain solid soil sample 1;

[0063] Add 3L of deionized water to the solid soil sample 1 to make the solid-liquid ratio 1:3,...

Embodiment 2

[0083] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the small molecule humic acid solution obtained by each dialysis in step e described in this embodiment needs to measure the dissolved total organic carbon content therein, if If the dissolved total organic carbon content is >1.0mg / L, replace the ultrapure water in the dialysis system again, add ultrapure water each time, stir for 20-40 hours, and measure the dissolved total organic carbon in the small molecule humic acid solution again , until the dissolved total organic carbon content in the small molecule humic acid solution is <0.5mg / L; then proceed to the operation of step f.

Embodiment 3

[0084] Example 3: The difference between this example and Example 1 is that this example also includes step h: after drying at 80-100°C for 20-40 hours, measure the soil size obtained in step g with a thermogravimetric analysis instrument. ash content of molecular humic acid components;

[0085] If the ash content is greater than 1.0%, based on dry weight, under the condition of nitrogen protection, use NaOH to dissolve the soil small molecule humic acid component, add deionized water to make the concentration of humic acid in the solution = 1-3g / L, and then Repeat steps f and g until the ash content of the small molecule humic acid component of the soil is less than 1.0%, on a dry weight basis.

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Abstract

The invention discloses a method for extracting small-molecule humic acid from soil through XAD resin. The method comprises the following steps of a, treating soil, b, extracting soil humic acid through sodium pyrophosphate, c, extracting soil humic acid through sodium hydroxide, d, removing fulvic acid impurities in the humic acid, e, separating large-molecule humic acid from small-molecule humic acid, f, removing silicon minerals from the humic acid, and g, performing concentration and extraction on the small-molecule humic acid. The method can well separate the small-molecule humic acid in the soil and provide sample security for the research on comprehensively understanding the environmental behavior of the humic acid.

Description

technical field [0001] The invention relates to the extraction of organic matter in soil, in particular to a method for extracting small molecule humic acid in soil by using XAD resin. Background technique [0002] Humic acid is a macromolecular organic mixture formed by animal and plant residues through complex physical, chemical, and biological processes. It widely exists in environmental media such as soil, water, and sediment. Humic acid can be dissolved in alkaline and neutral solution, insoluble in acid accounts for about 60-80% of the total soil organic matter, and is the most important component of soil organic matter. [0003] Small molecule humic acid refers to humic acid with a molecular weight less than 10,000 Daltons. There are two schools of thought among researchers on the geochemical effects of small molecule humic acid. One school of researchers believes that the structure of small molecule humic acid is relatively simple, and the degree of humification of...

Claims

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Application Information

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IPC IPC(8): G01N1/34
Inventor 吴丰昌白英臣廖海清陈曲宋凡浩
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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