Spina date seed reference extract and preparation method and application thereof

A technology of jujube seed and extract is applied in the field of reference extract of jujube seed and its preparation, which can solve the problems of single evaluation index, unenvironmental protection and high detection cost, and achieve the effects of comprehensive feedback component information, simple operation and low detection cost.

Active Publication Date: 2015-12-30
广州市药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this content determination method, the conventional traditional Chinese medicine quality control mode is still used, that is, jujube seed saponin A, B or spinosin reference substance is used as a contrast, and the quality evaluation analysis of jujube seed is carried out. The evaluation index of this mode Relatively single, overly dependent on traditional Chinese medicine chemical reference substances, not environmentally friendly, and high testing costs

Method used

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  • Spina date seed reference extract and preparation method and application thereof
  • Spina date seed reference extract and preparation method and application thereof
  • Spina date seed reference extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] A kind of Suanzaoren reference extract is prepared by the following method, and its technological process is as follows: figure 1 As shown, the details are as follows:

[0078] 1. To crush.

[0079] The Chinese date seed medicinal material is taken and pulverized to obtain the Chinese date seed coarse powder.

[0080] 2. Degreasing.

[0081] Get 1kg of the above-mentioned Suanzaoren coarse powder and place it in supercritical CO 2 In the extraction tank of the extraction device, the extraction is carried out according to the following conditions: the temperature of the extraction tank is 50°C, the pressure is 30Mpa, the temperature of the first separation tank is 45°C, the pressure is 6.0Mpa, the temperature of the second separation tank is 35°C, and the pressure is 4.5 MPa, CO 2 The flow rate is 30L / hour, and the extraction takes 3 hours.

[0082] The medicinal dregs are obtained after extracting and removing the lipid components in the coarse powder of Suanzaoren...

Embodiment 2

[0142] The wild jujube seed control extract prepared in the above-mentioned Example 1 was used in the identification and content determination of Zaoren Anshen Capsules and Zaoren Anshen Liquid.

[0143] 1. TLC identification.

[0144] 1. Preparation of the test article.

[0145] (1) Preparation of Zaoren Anshen Capsule and Zaoren Anshen Liquid for testing.

[0146] Zaoren Anshen Capsule: Take 4g of the content of this product, add 50ml of ether, heat and reflux for 30 minutes, filter, evaporate the solvent from the dregs, add 50ml of methanol, heat and reflux for 30 minutes, filter, evaporate the filtrate to dryness, add 20ml of water to the residue Dissolve, shake and extract 3 times with n-butanol saturated with water, 25ml each time, combine the n-butanol solution, wash with ammonia test solution twice, 10ml each time, take the n-butanol solution, evaporate to dryness, add 1ml methanol to the residue to make Dissolved as the test solution.

[0147] Zaoren Anshen Liquid:...

Embodiment 3

[0174] Suanzaoren formula granules are a single medicinal preparation of Suanzaoren, and there is currently no quality standard. The present invention refers to the established method of the above-mentioned Suanzaoren reference extract, respectively adopts the evaluation mode of the reference substance and the reference extract to evaluate the quality of the Suanzaoren formula granules in three aspects of TLC identification, fingerprint and content determination, and Compare the results of the two evaluation modes.

[0175] 1. TLC identification

[0176] 1. Preparation of the test article.

[0177] (1) Preparation of the test solution of Suanzaoren Formula Granules.

[0178] Take this product and grind it finely, take about 0.3g, add 20ml of methanol, heat to reflux for 1 hour, filter, evaporate the filtrate to dryness, add 1ml of methanol to the residue to dissolve, and use it as the test solution.

[0179] (2) The preparation of the reference extract solution of Suanzaore...

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Abstract

The invention relates to spina date seed reference extract and a preparation method and application thereof and belongs to the technical field of traditional Chinese medicine quality control. The preparation method of the spina date seed reference extract includes the following steps of smashing, degreasing, extracting, macroporous resin purifying, silica gel purifying and acquiring of the spina date seed reference extract finally. Spina date seed medicinal materials are degreased, extracted and purified through a specific method, finally the spina date seed reference extract with specific constituents is acquired, and the reference extract includes jujuboside A, jujuboside B and other saponin constituents and further includes spinosin, 6'''-ferula asafetida spinosin and other flavonoid constituents. The spina date seed reference extract serves as a reference to be used in quality control over a traditional Chinese medicine preparation containing spina date seed medicine flavor and has the advantage that constituent information is comprehensively fed back; when the spina date seed reference extract is used, the complex pretreatment step is not needed, inspection can be directly conducted after dissolution, and the advantages of being easy and convenient to operate and low in detection cost are achieved.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a reference extract of Jujube Semen and its preparation method and application. Background technique [0002] Suanzaoren, also known as Suanzaoren, Shanzaoren. It is the dry mature seed of the Rhamnaceae plant Zizyphus jujube. Harvest the mature fruit in late autumn and early winter, remove the pulp and core shell, collect the seeds, and dry them in the sun. It is born on sunny hillsides and roadsides, and is mainly produced in Henan, Hebei, Shaanxi, Liaoning, Shanxi, Shandong, Yunnan and other places. Harvest when the fruit is ripe in autumn, remove the pulp and hard core, take the seeds, and use raw or slightly fried. [0003] Suanzaoren, as a kind of traditional Chinese medicine, is recorded in the Chinese Pharmacopoeia. The Chinese Pharmacopoeia carried out thin-layer chromatography identification on the saponins and flavonoids of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/90
Inventor 林彤毕福钧谢培山侯惠婵江英桥
Owner 广州市药品检验所
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