Rhizoma corydalis reference extract and preparation method and application thereof

The technology of extract and Corydalis Corydalis, applied in the field of Corydalis Corydalis control extract and preparation thereof, can solve the problems of single evaluation index and high detection cost, and achieve the effects of comprehensive feedback component information, low detection cost and simple operation.

Active Publication Date: 2018-09-11
广州市药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this content determination method, the conventional traditional Chinese medicine quality control mode is still used, that is, at least one single-component reference substance is used as a contrast, and the quality evaluation and analysis of Corydalis Corydalis is carried out. costly defects

Method used

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  • Rhizoma corydalis reference extract and preparation method and application thereof
  • Rhizoma corydalis reference extract and preparation method and application thereof
  • Rhizoma corydalis reference extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A Corydalis Corydalis reference extract is prepared by the following method, and its technological process is as follows: figure 1 As shown, the details are as follows:

[0080] 1. Pretreatment.

[0081] Take Corydalis Corydalis and crush it into fine powder.

[0082] 2. Extraction.

[0083] Take 100g of the above Corydalis fine powder, put it in a thermostat, add 15 times the amount of 70% ethanol aqueous solution (1500ml) and heat to reflux, reflux twice, each time for 30min, let stand to cool, filter, and combine the filtrates.

[0084] 3. Concentration.

[0085] The ethanol was recovered from the filtrate under reduced pressure at 40°C until there was no alcohol smell (about 300 mL) to obtain a concentrated solution.

[0086] 4. Macroporous resin purification.

[0087] Above-mentioned concentrated solution has insoluble matter, concentrated solution adjusts its pH value to 9~10 with ammonia water, goes up pretreated D101 type macroporous adsorption resin column...

Embodiment 2

[0132] The Corydalis Corydalis control extract prepared in the above-mentioned Example 1 was used in the identification and content determination of Corydalis Corydalis Herbs, Vinegar Corydalis Corydalis Slices, Vinegar Corydalis Corydalis Formula Granules and Yuanhu Zhitong Tablets.

[0133] 1. TLC identification of Corydalis Corydalis and Corydalis Corydalis in Vinegar.

[0134] 1. Preparation of the test article.

[0135] (1) Preparation of the test solution.

[0136] The preparation of Corydalis Corydalis reference drug solution is the same as in Example 1.

[0137] Corydalis medicinal material and vinegar Corydalis decoction pieces: Take 1g of Corydalis herbal medicine and Corydalis Corydalis powder, add 50ml of methanol, ultrasonic treatment for 30 minutes, filter, evaporate the filtrate to dryness, add 10ml of water to the residue to dissolve, add concentrated ammonia test solution to adjust to alkaline, Shake and extract with diethyl ether 3 times, 10ml each time, co...

Embodiment 3

[0183] 1. Corydalis Corydalis control extract preparation.

[0184] A reference extract of Corydalis Corydalis, prepared according to the method of Example 1, the only difference is that the step of recovering the solvent under reduced pressure is recovered under reduced pressure at 30°C to obtain Corydalis Corydalis Control Extract E.

[0185] Second, detection.

[0186] HPLC detection was performed on Corydalis Corydalis Control Extract A prepared in Example 1 and Corydal Corydalis Corydalis Control Extract E prepared in this Example.

[0187] 1. Detection conditions.

[0188] Stationary phase: DIKMA-C 18 Chromatographic column (250×4.6mm, 5μm);

[0189] Column temperature: 25°C;

[0190] Mobile phase: Phase A is acetonitrile, phase B is 0.1% phosphoric acid water, and phase B is adjusted to pH 6.1 with triethylamine, and the gradient elution procedure is as follows:

[0191] Table 5. Gradient elution program

[0192]

[0193] Flow rate: 1.0ml / min;

[0194] Detecto...

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Abstract

The invention relates to a rhizoma corydalis reference extract and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine quality control. Thepreparation method of the rhizoma corydalis reference extract comprises the following steps of extraction, concentration, purification of macroporous resin, purification of alkaline aluminum oxide and obtaining of the rhizoma corydalis reference extract at last. The rhizoma corydalis medicinal materials are extracted and purified by adopting a specific method, and finally the rhizoma corydalis reference extract containing the specific ingredients is obtained. The extract is prepared from various kinds of rhizoma corydalis index components, the rhizoma corydalis reference extract obtained through the preparation method serves as a comparison and is used in quality control of rhizoma corydalis and traditional Chinese medicinal preparation containing rhizoma corydalis, and has the advantageof feeding back the component information comprehensively, meanwhile, the rhizoma corydalis reference extract serves a comparison, tedious pre-treatment step does not need to be conducted, detection can be directly conducted after the rhizoma corydalis reference extract is dissolved, and the rhizoma corydalis reference extract has the advantages that operation is easy and convenient, and the detection cost is low.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a Corydalis Corydalis reference extract and its preparation method and application. Background technique [0002] Corydalis yanhusuo (Corydalis yanhusuoW.T.Wang), also known as: Yanhu, Xuanhusuo, Yuanhusuo, Yuanhu, etc. It is a perennial herb of Papaveraceae and Corydalis genus. The tuber is a well-known commonly used traditional Chinese medicine, containing more than 20 kinds of alkaloids. [0003] Corydalis Corydalis, as a traditional Chinese medicine, is recorded in the Chinese Pharmacopoeia. For the identification of Corydalis Corydalis, the provisions in the Chinese Pharmacopoeia are: take 1g of this product powder, add 50ml of methanol, ultrasonic treatment for 30 minutes, filter, evaporate the filtrate to dryness, add 10ml of water to the residue to dissolve, add concentrated ammonia test solution to adjust to alkaline , Shake and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/04
CPCG01N30/04G01N2030/042
Inventor 江英桥林彤毕福钧吕渭升王欢侯惠婵
Owner 广州市药品检验所
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