Corydalis control extract and its preparation method and application

The technology of extract and Corydalis Corydalis, which is applied in the field of Corydalis Corydalis control extract and its preparation, can solve the problems of single evaluation index and high detection cost, and achieve the effects of comprehensive feedback component information, low detection cost and simple operation.

Active Publication Date: 2021-03-16
广州市药品检验所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this content determination method, the conventional traditional Chinese medicine quality control mode is still used, that is, at least one single-component reference substance is used as a contrast, and the quality evaluation and analysis of Corydalis Corydalis is carried out. costly defects

Method used

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  • Corydalis control extract and its preparation method and application
  • Corydalis control extract and its preparation method and application
  • Corydalis control extract and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A Corydalis Corydalis control extract is prepared by the following method, and its technological process is as follows figure 1 shown, as follows:

[0080] 1. Pretreatment.

[0081] Take Corydalis and pulverize it into fine powder.

[0082] 2. Extract.

[0083] Take 100 g of the above-mentioned Corydalis Corydalis fine powder, put it in a thermostat, add 15 times the amount of 70% ethanol aqueous solution (1500ml), heat to reflux, reflux twice, 30min each time, stand to cool, filter, and combine the filtrates.

[0084] 3. Concentrate.

[0085] The filtrate was recovered under reduced pressure at 40° C. until no alcohol smell (about 300 mL) was obtained to obtain a concentrated solution.

[0086] 4. Macroporous resin purification.

[0087] The above-mentioned concentrated solution has insoluble matter, and the pH value of the concentrated solution is adjusted to 9-10 with ammonia water, and together with the insoluble matter, it is placed on a pretreated D101 type m...

Embodiment 2

[0132] The Corydalis Corydalis control extract prepared in the above Example 1 was used in the identification and content determination of Corydalis Corydalis medicinal materials, Vinegar Corydalis decoction pieces, Vinegar Corydalis Formula Granules and Yuanhu Zhitong Tablets.

[0133] 1. Thin-layer identification of Corydalis Corydalis medicinal materials and vinegar Corydalis Corydalis pieces.

[0134] 1. Preparation of the test product.

[0135] (1) Preparation of the test solution.

[0136] The preparation of Corydalis Corydalis reference medicinal material solution is the same as that in Example 1.

[0137] Corydalis Corydalis and Vinegar Corydalis Decoction Pieces: Take 1 g each of Corydalis Corydalis and Vinegar Corydalis Decoction Pieces, add 50 ml of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 10 ml of water to the residue to dissolve, add concentrated ammonia test solution to make it alkaline, Shake and extract 3 t...

Embodiment 3

[0182] 1. Preparation of the control extract of Corydalis Corydalis.

[0183] A reference extract of Corydalis chinensis was prepared according to the method of Example 1, the only difference being that the steps of recovering the solvent under reduced pressure were all recovered under reduced pressure at 30° C. to obtain the reference extract E of Corydalis Corydalis.

[0184] 2. Detection.

[0185] HPLC detection was carried out on the Corydalis chinensis control extract A prepared in Example 1 and the Corydalis Corydalis control extract E prepared in this example.

[0186] 1. Detection conditions.

[0187] Stationary Phase: DIKMA-C 18 Chromatography column (250×4.6mm, 5μm);

[0188] Column temperature: 25℃;

[0189] Mobile phase: phase A is acetonitrile, phase B is 0.1% phosphoric acid water, and phase B is adjusted to pH 6.1 with triethylamine, according to the following gradient elution procedure:

[0190] Table 4. Gradient elution procedure

[0191]

[0192] Flo...

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Abstract

The invention relates to a rhizoma corydalis reference extract and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine quality control. Thepreparation method of the rhizoma corydalis reference extract comprises the following steps of extraction, concentration, purification of macroporous resin, purification of alkaline aluminum oxide and obtaining of the rhizoma corydalis reference extract at last. The rhizoma corydalis medicinal materials are extracted and purified by adopting a specific method, and finally the rhizoma corydalis reference extract containing the specific ingredients is obtained. The extract is prepared from various kinds of rhizoma corydalis index components, the rhizoma corydalis reference extract obtained through the preparation method serves as a comparison and is used in quality control of rhizoma corydalis and traditional Chinese medicinal preparation containing rhizoma corydalis, and has the advantageof feeding back the component information comprehensively, meanwhile, the rhizoma corydalis reference extract serves a comparison, tedious pre-treatment step does not need to be conducted, detection can be directly conducted after the rhizoma corydalis reference extract is dissolved, and the rhizoma corydalis reference extract has the advantages that operation is easy and convenient, and the detection cost is low.

Description

technical field [0001] The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a reference extract of Corydalis Corydalis and a preparation method and application thereof. Background technique [0002] Corydalis yanhusuo W.T.Wang, also known as: Yanhu, Xuanhusuo, Yuanhusuo, Yuanhu, etc. It is a perennial herb of the family Papaveraceae and Viola, and its tubers are well-known and commonly used in traditional Chinese medicine. [0003] As a kind of Chinese herbal medicine, Corydalis is included in the Chinese Pharmacopoeia. For the identification of Corydalis Corydalis, the Chinese Pharmacopoeia stipulates: take 1g of this product powder, add 50ml of methanol, ultrasonically treat it for 30 minutes, filter, evaporate the filtrate to dryness, add 10ml of water to the residue to dissolve, add concentrated ammonia test solution to make it alkaline , Shake and extract 3 times with ether, each 10ml, combine the ether sol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/04
CPCG01N30/04G01N2030/042
Inventor 江英桥林彤毕福钧吕渭升王欢侯惠婵
Owner 广州市药品检验所
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