Rapid detection method for alkannin
A detection method, shikonin technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex sample processing process and long overall analysis time, and achieve the goals of improving analytical sensitivity, rapid separation, rapid and accurate detection Effect
Inactive Publication Date: 2015-12-30
XIAN UNIV OF SCI & TECH
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The sample processing process of this analytical method is complex and the overall analysis time is long
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[0023] A method for detecting shikonin based on PY-GC / MS, taking the Chinese herbal medicine Zicao as an example, but the protection scope of the present invention is not limited to this embodiment, and the specific steps are as follows:
[0024] 1. Sample preparation
[0025] Accurately weigh 1.00 mg (±0.02 mg) of the obtained Xinjiang comfrey (Shaanxi Yongyuan Biotechnology Co., Ltd., Xi'an) into a 50 μL pyrolysis cuvette;
[0026] 2. Pyrolysis:
[0027] The pyrolysis furnace adopts Frontiers PY2020is;
[0028] ①Pyrolysis temperature: 280°C;
[0029] ②Pyrolysis time: 0.20min.
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Abstract
The invention provides a rapid detection method for alkannin. The method comprises the steps of 1, sample preparation, 2, PY-GC / MS detection and 3, data analysis and qualitative and quantitative analysis. The PY-GC / MS technology is selected for detecting the content of alkannin in lithospermum, the rapid and microcrystalline detection method for alkannin is provided for solving the problems that qualities of the alkannin in actual production and living are different due to different producing areas, seasons and processing methods, and then the alkannin or the quality of the alkannin compound preparation is preliminarily judged. The rapid detection method has the advantages of being easy to implement, low in detection limit, good in experiment reproducibility and visual and reliable in experimental data, is particularly applicable to micro-sample detection, and can be used for preliminary comparison of quality differences of alkannin or alkannin compound preparation samples of different producing areas, varieties, batches and manufacturers, the application range is wide, and the preliminary judgment basis is provided for quality standard and modernization application of alkannin or alkannin compound preparations.
Description
technical field [0001] The invention relates to a method for rapidly detecting shikonin content based on fast pyrolysis-gas chromatography-mass spectrometry (PY-GC / MS method). Background technique [0002] Comfrey is one of the traditional Chinese medicines in my country. Its root is used as medicine, which has the functions of cooling blood, promoting blood circulation, and detoxifying rashes. It mainly contains shikonin, acetylIshikonin, and other tea phenolic compounds. Has a wide range of pharmacological effects. The anti-inflammatory and anti-tumor effects of comfrey are very obvious, and it is often used in folks to treat burns, ulcers and various cancers. Among them, shikonin is an important and main active ingredient of this type of traditional Chinese medicine, and its structural feature is a naphthoquinone compound. The results of pharmacological studies have also confirmed that shikonin has obvious anti-inflammatory, anti-fungal and anti-tumor effects. It has be...
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Inventor 陈福欣龚频周安宁赵伟
Owner XIAN UNIV OF SCI & TECH