Multi-ring test sheet for rapid microbial detection, aseptic culture medium and preparation method of aseptic culture medium

A technology for detecting and testing microorganisms, applied in the field of microbial testing, can solve the problems of insufficient mixing of medium components and water, high cost of use, uneven nutrition, etc., and achieve the effects of facilitating growth identification, low testing cost, and long shelf life.

Active Publication Date: 2016-01-06
北京万通天元科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The ready-to-use culture medium is divided into ready-to-use liquid enrichment solution and ready-to-use agar plate, agar slant, etc., which have the problems of inconvenient transportation and high use cost.
And most of the existing culture medium contains agar-like substances, and most of the current test sheets use water-absorbing gel or cold water gel, and some are used in conjunction with water-absorbing filter paper. When in use, as described in the above-mentioned published patent, add water Like this, it is absorbed by gel or water-absorbing filter paper, that is, it is used for cultivation, and it is easy to cause insufficient mixing of medium components and water, and uneven nutrition. solved problem

Method used

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  • Multi-ring test sheet for rapid microbial detection, aseptic culture medium and preparation method of aseptic culture medium
  • Multi-ring test sheet for rapid microbial detection, aseptic culture medium and preparation method of aseptic culture medium
  • Multi-ring test sheet for rapid microbial detection, aseptic culture medium and preparation method of aseptic culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0063] refer to figure 1 , 2 , 3, 4, and 5, in an exemplary embodiment of the present invention, a rapid microbial detection test piece includes a base plate 1, a loam cake 2 and a sterile medium; the base plate 1 and loam cake 2 are The multi-ring tooth-like structure adapts to the shape and can be occluded up and down. The multi-ring tooth-like structure is a continuous structure that undulates up and down; the middle part of the multi-ring tooth-like structure of the bottom plate 1 is sunken to form the cultivation chamber 3. For the convenience of description, the cultivation chamber 3 is divided into a bottom surface 31 and a side surface 32; correspondingly, the middle part of the multi-ring tooth structure of the upper cover 2 protrudes to form a culture chamber cover 4; the angle between the bottom surface 31 of the culture chamber and the side surface 32 is 30-80°, and the highest point of the side surface 32 is vertical The height of the bottom surface 31 is not les...

Embodiment 2

[0095] refer to figure 1 , 2 , 6, in an exemplary embodiment of the present invention, a rapid microbial detection test piece, including a base plate 1, a loam cake 2 and a sterile medium; the base plate 1 and loam cake 2 are adapted in shape, A multi-ring tooth-like structure that can engage up and down, the multi-ring tooth-like structure is a continuous structure that undulates up and down; the middle part of the multi-ring tooth-like structure of the bottom plate 1 is recessed to form the cultivation chamber 3; correspondingly, the multi-ring tooth-like structure of the upper cover 2 The middle part of the structure protrudes to form a culture chamber cover 4; the angle between the bottom surface 31 of the culture chamber and the side surface 32 is 30-80°, and the height of the highest point of the side surface 32 perpendicular to the bottom surface 31 is not less than 0.3cm; the culture chamber 3 is used to place sterile Culture medium, the inner surface of the bottom su...

Embodiment 3

[0114] refer to figure 1 , 2 , 7, in an exemplary embodiment of the present invention, a rapid detection test piece for microorganisms, including a base plate 1, an upper cover 2 and a sterile medium; the base plate 1 and the upper cover 2 are adapted in shape, A multi-ring tooth-like structure that can be occluded up and down. The multi-ring tooth-like structure is a continuous structure that undulates up and down; the middle part of the multi-ring tooth-like structure of the bottom plate 1 is recessed to form the cultivation chamber 3. For the convenience of description, the cultivation chamber 3 is divided into the bottom surface 31 and side 32; Correspondingly, the middle part of the multi-ring tooth structure of loam cake 2 protrudes to form the culture chamber cover 4; The height is not less than 0.3cm; the cultivation chamber 3 is used to place sterile medium; the sterile medium is a gel-separated sterile medium; the gel-separated sterile medium includes sterile high-c...

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Abstract

The invention relates to a multi-ring test sheet for rapid microbial detection, an aseptic culture medium contained in the test sheet and a preparation method of the aseptic culture medium and belongs to the technical field of microbial inspection. The multi-ring test sheet comprises a bottom board, an upper cover and the aseptic culture medium. The bottom board and the upper cover are in multi-ring toothed structures having fitting shapes and allowing top-bottom engagement. The middle of the multi-ring toothed structure of the bottom board is recessed to form a culture chamber; correspondingly, the middle of the multi-ring toothed structure of the upper cover is raised to form a culture chamber cover. The bottom of the culture chamber is 30-80 degrees angled to its side, a highest point of this side is not less than 0.3 cm perpendicular to the bottom, the culture chamber is used for holding the aseptic culture medium, and the aseptic culture medium is gel separate aseptic culture medium or electrostatic absorption culture medium. The detection is simple and quick, the multi-ring toothed structures are effective in keeping the medium free of moisture loss, gaseous ingredients required by microbial growth are provided, and the development of microbial detection is facilitated.

Description

technical field [0001] The invention relates to a multi-ring test piece for rapid detection of microorganisms, an aseptic medium contained in the test piece, and a preparation method of the aseptic medium, belonging to the technical field of microbial inspection. Background technique [0002] Rapid test strips are a rapid microbial detection method developed in recent years. The test strips with filter paper as the carrier and Petrifilm as the carrier have been recognized by many inspection departments. For the test piece with filter paper as the carrier, because the medium and the carrier are dissolved as one, it is inconvenient to pick colonies for further isolation and identification of microorganisms growing between the filter paper and the bottom plate. The colonies outside the edge of the circular culture area and outside the edge of the test piece with Petrifilm as the carrier may be brought in by bacteria in the air under certain culture conditions, resulting in inac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/04
CPCC12M23/04C12M41/00C12Q1/04C12Q1/045
Inventor 原春锋
Owner 北京万通天元科技有限责任公司
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